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网站主页 化工产品目录 生物化工 抑制剂 表观遗传学(Epigenetics) Epigenetic Reader Domain 抑制剂 (E)-4-((2-AMINO-4-HYDROXY-5-METHYLPHENYL)DIAZENYL)-N-(PYRIDIN-2-YL)BENZENESULFONAMIDE 化合物 MS436
  • 化合物 MS436|T1854|TargetMol

化合物 MS436|T1854|TargetMol

MS436
1395084-25-9
￥266 1mg 起订
￥378 2mg 起订
￥622 5mg 起订
上海 更新日期：2024-09-29

TargetMol中国（陶术生物）

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联系人：邵小姐
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邮箱：marketing@tsbiochem.com

产品详情:

中文名称：
化合物 MS436
英文名称：
MS436
CAS号：
1395084-25-9
品牌：
TargetMol
产地：
美国
保存条件：
Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
纯度规格：
98.92%
产品类别：
抑制剂
货号：
T1854

Product Introduction

Bioactivity

NameMS436
DescriptionMS436 is a selective, small-molecule inhibitor for the BRD4 bromodomains.
Cell ResearchMurine macrophage RAW264.7 cells are plated at a density of 10000 cells per well in a 96-well plate and incubated at 37 °C for 18 h. The cells are then treated with the diazobenzene bromodomain inhibitors up to 100 μL for 24 hours. At the end of the 24 hr incubation, 10 μL of the MTT solution (4 mg/ml) is added to each well and incubated at 37°C for 4 h. The supernatants are then removed and the cells were solubilized in 100 μL of 100% DMSO. The diazobenzene compounds are first dissolved in DMSO then diluted with culture medium to concentrations that ranged from 0.28 to 50000 nM. The final concentration of DMSO is adjusted to 0.05% (v/v). The extent of the reduction is measured by the absorbance at 570/630 nm using EnVison 2104 Multilabel Reader.(Only for Reference)
Kinase AssayFluorescence Anisotropy Binding Assay: Binding affinity of the newly synthesized diazobenzene compounds for various bromodoamins is assessed in a fluorescence anisotropy competition assay using a fluorescein isothiocyanate (FITC)-labeled MS417 as an assay probe. Competition experiments are performed with a BrD protein (0.25–1 μM) and the fluorescent probe (80 nM), and increasing concentration of unlabeled competing ligand in a PBS buffer (pH 7.4) in total volume of 80 μL Measurements are obtained after a 1 hour incubation of the fluorescent ligand and the protein at 25°C with Safire 2 microplate reader. In a competition-binding assay, fluorescent ligand concentration is ≤ 2Kd, and protein concentration was set at which 50-80% of fluorescent ligand is bound. Dissociation constant of a competing ligand is calculated with the correction to Cheng-Prussoff equation introduced by Nicolovska-Coleska and colleagues. Assuming one-site competitive binding model, the equation used to calculate Ki's from IC50 values recovered from fitting data using Prism.
In vivo对于第一溴结构域蛋白,MS436表现出低纳摩尔亲和力（Ki=30-50 nM）。在NF-κB定向产生的小鼠巨噬细胞中,MS436能够抑制NO和IL-6的BRD4活性。
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility InformationDMSO : 40 mg/mL (104.32 mM)
KeywordsInhibitor | MS436 | inhibit | Epigenetic Reader Domain | MS 436 | MS-436
Inhibitors RelatedBisdemethoxycurcumin | dBET6 | 3-methyl-1,2,3,4-tetrahydroquinazolin-2-one | CeMMEC1 | (+)-JQ-1 | Curcumin | Naphthol AS-E | J-147 | ABBV-744 | Piflufolastat | Hexamethylene bisacetamide | Anacardic Acid
Related Compound LibrariesInhibitor Library | Bioactive Compound Library | Bioactive Compounds Library Max | Autophagy Compound Library | Chromatin Modification Compound Library | Histone Modification Compound Library | Anti-Aging Compound Library | Epigenetics Compound Library | PPI Inhibitor Library | Target-Focused Phenotypic Screening Library
MS436|TargetMol

公司简介

TargetMol Chemicals Inc. 总部位于马萨诸塞州波士顿，致力于为全球生化领域科学家的研究提供专业的产品和服务。TargetMol?品牌的客户群分布于40多个国家和地区，已发展成为全球知名的化合物库和小分子化合物研究供应商。 TargetMol?可提供160多种满足不同需求的化合物库，以及多种类型的生化试剂产品，包括12000多种抑制剂、16000多种天然产物和各类多肽、抗体、生命科学试剂盒等，此外，我们还建设有CADD（计算机辅助药物设计）研究中心、药理实验室、药化合成平台三大技术中心，全方位满足客户的定制需求。 凭借我们优质的产品和服务、快速高效的全球供应链和专业的技术支持，我们将有效帮助您缩短研发周期，取得更成功的结果。

成立日期 (12年)
注册资本 566.265100万人民币
员工人数 100-500人
年营业额 ￥ 1亿以上
经营模式 贸易,工厂,试剂,定制,服务
主营行业 天然产物,生化试剂,分子生物学,分子砌块,生物技术服务

化合物 MS436相关厂家报价

  • MS436
  • MS436
  • 深圳盛大医药科技有限公司
  • 2023-03-15
  • ￥2113
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