化合物 RI1|T2276|TargetMol

RI-1
415713-60-9

￥266 1mg 起订

￥378 2mg 起订

￥622 5mg 起订

上海更新日期：2024-12-12

TargetMol中国（陶术生物）

VIP3年

联系人：邵小姐

手机：4008200310 拨打

邮箱：marketing@tsbiochem.com

产品详情:

中文名称：: 化合物 RI1

英文名称：: RI-1

CAS号：: 415713-60-9

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.62%

产品类别：: 抑制剂

货号：: T2276

Product Introduction

Bioactivity

名称	RI-1
描述	RI-1 (RAD51 inhibitor 1) is a RAD51 inhibitor (IC50: 5-30 μM).
细胞实验	Cytotoxicity is determined by loss of colony-forming ability. Experiments are performed in triplicate. Crystal violet stained colonies are imaged with a CCD camera and counted using NIH Image software. Error bars denote standard error.(Only for Reference)
激酶实验	DNA binding assays: All reactions are performed in black non-binding polystyrene 384-well plates with reaction volumes of 30–100 μL. Purified DNA strand exchange proteins and chemical compounds are pre-incubated at room temperature for 5 minutes; they are then further incubated at 37°C for 30 min with 100 nM of ssDNA substrate, consisting of a 45-mer poly-dT tagged with Alexa 488 at the 5' terminus (synthesized and purified by Integrated DNA Technologies). Reactions are performed in 20 mM HEPES pH 7.5, 10 mM MgCl2, 0.25 μM BSA, 2% glycerol, 30 mM NaCl, 4% DMSO and 2 mM ATP. Some conditions included DTT or TCEP (tris(2-carboxyethyl)phosphine) as indicated. DNA binding is measured as a function of fluorescence polarization (FP) with a Safire2 plate reader, using the following settings: excitation 470±5 nM, emission 530±5 nM, 10 reads/well, Z height and G factor auto-calibrated from control wells. Displayed error bars represent standard deviation. For experiments involving a titration of protein concentrations, data are fit to an equation that accounts for the cooperative nature by which recombinase proteins bind DNA. For experiments involving a titration of RI-1, protein concentrations are selected to give an ～80% saturation of the FP signal in the absence of RI-1.
体内活性	在HeLa细胞,MCF-7和U2OS三种癌细胞株中,RI-1具有单剂毒性（LD50：20-40 μM）。通过直接和特异破坏HsRAD51和抑制RAD51,RI-1可抑制RAD51在ssDNA上形成丝的能力,从而使细胞对DNA损伤的敏感性增加。RI-1使G2期细胞的γ-H2AX灶减少,并使辐射6小时后未修复DSB的水平增加。
存储条件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	DMSO : 36.2 mg/mL (100 mM)
关键字	recombination \| homologous \| RI 1 \| Inhibitor \| RAD51 \| inhibit \| RI-1
相关产品	N-Nitrosodiethylamine \| Acyclovir \| Guanidine hydrochloride \| Trimethoprim \| Procaine \| Pemetrexed \| Folic acid \| Resveratrol \| 5-Fluorouracil \| Temozolomide \| Thymidine \| Ribavirin
相关库	抑制剂库 \| 经典已知活性库 \| 抗癌化合物库 \| 已知活性化合物库 \| 细胞周期化合物库 \| 抗衰老化合物库 \| NO PAINS 化合物库 \| DNA 损伤和修复分子库 \| 毒性化合物库

RI-1; 3-氯-1-(3,4-二氯苯基)-4-(4-吗啉基)-1H-吡咯-2,5-二酮|||RI1|||RAD51 inhibitor 1|TargetMol

TargetMol Chemicals Inc. 总部位于马萨诸塞州波士顿，致力于为全球生化领域科学家的研究提供专业的产品和服务。TargetMol?品牌的客户群分布于40多个国家和地区，已发展成为全球知名的化合物库和小分子化合物研究供应商。 TargetMol?可提供160多种满足不同需求的化合物库，以及多种类型的生化试剂产品，包括12000多种抑制剂、16000多种天然产物和各类多肽、抗体、生命科学试剂盒等，此外，我们还建设有CADD（计算机辅助药物设计）研究中心、药理实验室、药化合成平台三大技术中心，全方位满足客户的定制需求。凭借我们优质的产品和服务、快速高效的全球供应链和专业的技术支持，我们将有效帮助您缩短研发周期，取得更成功的结果。

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