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  • 化合物 BV6|T6428|TargetMol

化合物 BV6|T6428|TargetMol

BV6
1001600-56-1
373 1mg 起订
497 2mg 起订
675 5mg 起订
上海 更新日期:2024-09-29

TargetMol中国(陶术生物)

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联系人:邵小姐
手机:4008200310 拨打
邮箱:marketing@tsbiochem.com

产品详情:

中文名称:
化合物 BV6
英文名称:
BV6
CAS号:
1001600-56-1
品牌:
TargetMol
产地:
美国
保存条件:
Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
纯度规格:
99.89%
产品类别:
抑制剂
货号:
T6428

Product Introduction

Bioactivity

名称BV6
描述BV6 is an antagonist of c-IAP1 and XIAP, members of the inhibitors of apoptosis (IAP) family.
细胞实验BV6 is prepared in DMSO and stored, and then diluted with appropriate medium before use. H460 and HCC193 cell lines are cultured in RPMI-1640 supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. Cell viability is measured using the CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Assay kit. 5000 cells/well are seeded into 96-well plates in triplicate. Following adhesion of cells to the wells, increasing concentrations of BV6 are added into different wells. Control groups are exposed to the same concentration of DMSO. The final concentrations of 333 μg/mL MTS and 25 μM PMS are added to each well 24 hours later. After two hours incubation at 37°C in humidified 5% CO2, plates are read at the absorbance of 490 nm on a microplate reader. Relative cell viability of an individual sample is calculated by normalizing their absorbance to that of the corresponding control. IC50 values are calculated using Prism 5.01. For the TNFα neutralizing antibody assay, cells are exposed to 1 and 5 μM BV6 with or without 10 μg/mL Infliximab and the assay is performed 24 hours later. Plates are read at the absorbance of 490 nm on a microplate reader.
动物实验BV6 is prepared in DMSO and diluted with saline or PBS. Female mice (6 weeks of age, BALB/c) are used. All 24 mice are ovariectomized through a 1 cm longitudinal skin incision then injected s.c. with estradiol valerate (0.5 μg/mouse/week) once per week for 6 weeks until the experimental endometriosis induction. Two weeks after ovariectomy, the uteri of an additional eight donor mice (n=8) are removed en bloc after euthanasia and cleaned of excess tissue in sterile saline. Each uterus is cut to include the uterine horns in each half with a linear incision longitudinally and minced (0.5 mm in diameter) with dissecting scissors. The ovariectomized recipient mice (n=16) are anesthetized using pentobarbital sodium. A 0.5 cm subabdominal midline incision is made. Each recipient receives half of the donor uterus (1:2 donor uterus to host ratio) minced and added to 500 μl saline, and injected into the peritoneal cavity, and the peritoneum is sutured. Injected uterine tissue weighed ~50 mg per mouse. For the next 4 weeks, recipient mice are treated with a single i.p. injection of BV6 (n=8; 10 mg/kg) or vehicle (n=8; 1% DMSO) twice weekly.
体外活性对HCC193细胞处理1 μM BV6 24小时,相对于DMSO处理的细胞,HCC193细胞的生存曲线显著偏移,DER=1.38 (p<0.05)。BV6 (2和5 μM) 显著抑制异位和原位(无病和肌瘤)ESC中的BrdU合成。在使用5 μM BV6处理后,两组中BrdU合成均约减少30%。向HCC193细胞施加1 μM BV6后,治疗1小时即完全耗尽cIAP1水平,而XIAP水平减少则在化合物添加24小时后才出现。同样,5 μM BV6在1小时内完全耗尽H460细胞中的c-IAP1,并在24小时后开始减少XIAP。与此平行的发现是,对于两种细胞系,0.25 μM BV6的小剂量就能减少c-IAP1水平,而在5 μM BV6时,仍可观察到少量的XIAP。从与1 μM BV6孵育12小时后开始,HCC193细胞显著出现切割型caspase-3水平,且这一水平在48小时内随时间增长而持续增加。
体内活性小鼠c-IAP-1、c-IAP-2和XIAP表达在植入体的上皮细胞和基质细胞的细胞质中清晰可见,而Survivin主要表达在核内。经过4周的BV6处理后,IAPs表达的强度减弱。免疫组化染色后,角蛋白和波形蛋白呈阳性染色,而钙结合蛋白阴性。采用BV6治疗4周后,病变的总数(4.6对2.8/只小鼠)、平均重量(78.1对32.0 mg/只小鼠)和表面积(44.5对24.6 mm2/只小鼠)显著低于对照组。在子宫内膜腺上皮或基质中,Ki67阳性细胞的百分比在BV6治疗后降低。
存储条件Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
溶解度DMSO : 55 mg/mL (45.62 mM)
关键字inhibit | Inhibitor | BV6 | BV 6 | IAP | BV-6
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相关库经典已知活性库 | 已知活性化合物库 | 细胞凋亡化合物库 | NO PAINS 化合物库 | PPI抑制剂库
BV6|TargetMol

公司简介

TargetMol Chemicals Inc. 总部位于马萨诸塞州波士顿,致力于为全球生化领域科学家的研究提供专业的产品和服务。TargetMol?品牌的客户群分布于40多个国家和地区,已发展成为全球知名的化合物库和小分子化合物研究供应商。 TargetMol?可提供160多种满足不同需求的化合物库,以及多种类型的生化试剂产品,包括12000多种抑制剂、16000多种天然产物和各类多肽、抗体、生命科学试剂盒等,此外,我们还建设有CADD(计算机辅助药物设计)研究中心、药理实验室、药化合成平台三大技术中心,全方位满足客户的定制需求。 凭借我们优质的产品和服务、快速高效的全球供应链和专业的技术支持,我们将有效帮助您缩短研发周期,取得更成功的结果。

成立日期 (12年)
注册资本 566.265100万人民币
员工人数 100-500人
年营业额 ¥ 1亿以上
经营模式 贸易,工厂,试剂,定制,服务
主营行业 天然产物,生化试剂,分子生物学,分子砌块,生物技术服务

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