化合物 SirReal2|T6984|TargetMol

SirReal2
709002-46-0

￥298 1mg 起订

￥688 5mg 起订

￥987 10mg 起订

上海更新日期：2024-09-29

TargetMol中国（陶术生物）

VIP3年

联系人：邵小姐

手机：4008200310 拨打

邮箱：marketing@tsbiochem.com

产品详情:

中文名称：: 化合物 SirReal2

英文名称：: SirReal2

CAS号：: 709002-46-0

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.75%

产品类别：: 抑制剂

货号：: T6984

Product Introduction

Bioactivity

Name	SirReal2
Description	SirReal2 is a potent and selective Sirt2 inhibitor with IC50 of 140 nM.
Cell Research	HeLa cells were plated in petri dishes (5?cm), incubated overnight to a confluency of 30–40% and then treated with SirReal2 dissolved in RPMI1640 medium supplemented with fresh 20% (v/v) FCS, 1% (v/v) penicillin, 1% (v/v) streptomycin, 1% (v/v), L-glutamine, 1% (v/v) DMSO for 5?h at various concentrations. Cells were then washed with prewarmed PBS (2?ml), lysed in SDS–PAGE sample buffer (70?μl, 50?mM Tris/HCl, 0.5?mM EDTA, 1 × Complete Protease Inhibitors, 2% (v/v) IGEPAL, 2% (w/v) SDS, 10% (v/v) glycerol, 50?mM NCA, 3.3?μM trichostatin A, 50?mM DTT, 0.01% (w/v) bromophenol blue, pH 6.8) and sonicated (5?min). Cell samples were then separated using SDS–PAGE (12.5% (w/v) polyacrylamide), transferred to an activated nitrocellulose membrane (Bio-Rad), blocked with non-fat dry milk (Roth, 5% (w/v), TBS, 0.1% (v/v) Tween 20) and probed with an anti-acetyl-α-tubulin antibody (1:1,000) and an anti-GAPDH antibody (1:2,000–1:5,000) as a loading control.
Kinase Assay	Wee1 Mass Screening: Wee1 mass screening is performed using Amersham's p34cdc2 kinase SPA kit with some modifications. Briefly, 45–60 nM full-length Wee1 kinase is incubated with 25 μM compounds, 20 μM ATP, and 122–441 nM Cdc2/cyclin B in a final volume of 50 μl of enzyme dilution buffer [50 mM Tris (pH 8.0), 10 mM NaCl, 10 mM MgCl2, 1 mM DTT, and 0.1 mM Na3VO4]. After 30 min incubation at 30°C, 30 μl of [33P]ATP containing kinase buffer [67 mM Tris (pH 8.0), 40 mM NaCl, 13 mM MgCl2, 1 mM DTT, and 0.13 mM Na3VO4] containing 1 μM biotinylated peptide, and 0.25 μCi of [γ-33P]ATP is added to the reaction and incubated for another 30 min at 30°C. The reaction is stopped by adding 200 μl of stop buffer [50 μM ATP, 5 mM EDTA, 0.1% Triton X-100, and 1.25 mg/ml SPA beads in PBS]. After centrifugation at 2400 rpm for 15 min, the plate is counted with Wallac's Microbeta counter.
In vitro	SirReal2 selectively inhibits Sirt2 via a Sirt2-specific amino acid network, and induces a tubulin hyperacetylation and a significant depletion of BubR1 in HeLa cells. [1]
In vivo	In vivo, SirReal2 inhibits Sirt2 activity without affecting the activity of the other Class-I sirtuins Sirt1 and Sirt3. [1]
Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
Solubility Information	DMSO : 55 mg/mL (130.78 mM), Sonication is recommended.
Keywords	Sirtuin \| Inhibitor \| SirReal2 \| inhibit \| SirReal 2 \| SirReal-2
Inhibitors Related	Fisetin \| Salvianolic acid B \| Nicotinamide riboside \| Nicotinamide \| Resveratrol \| Dihydrocoumarin \| Sirtinol \| JGB1741 \| SRT 2104 \| 3-TYP \| SIRT6-IN-5 \| MC3482
Related Compound Libraries	Inhibitor Library \| Bioactive Compound Library \| Anti-Cancer Compound Library \| Bioactive Compounds Library Max \| Histone Modification Compound Library \| Anti-Aging Compound Library \| Highly Selective Inhibitor Library \| Glutamine Metabolism Compound Library \| Microtubule-Targeted Compound Library \| Target-Focused Phenotypic Screening Library

SirReal2|TargetMol

TargetMol Chemicals Inc. 总部位于马萨诸塞州波士顿，致力于为全球生化领域科学家的研究提供专业的产品和服务。TargetMol?品牌的客户群分布于40多个国家和地区，已发展成为全球知名的化合物库和小分子化合物研究供应商。 TargetMol?可提供160多种满足不同需求的化合物库，以及多种类型的生化试剂产品，包括12000多种抑制剂、16000多种天然产物和各类多肽、抗体、生命科学试剂盒等，此外，我们还建设有CADD（计算机辅助药物设计）研究中心、药理实验室、药化合成平台三大技术中心，全方位满足客户的定制需求。凭借我们优质的产品和服务、快速高效的全球供应链和专业的技术支持，我们将有效帮助您缩短研发周期，取得更成功的结果。

成立日期	(12年)
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