化合物 TRAM34|T6186|TargetMol

TRAM-34
289905-88-0

￥233 2mg 起订

￥396 5mg 起订

￥619 10mg 起订

上海更新日期：2024-09-29

TargetMol中国（陶术生物）

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联系人：邵小姐

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邮箱：marketing@tsbiochem.com

产品详情:

中文名称：: 化合物 TRAM34

英文名称：: TRAM-34

CAS号：: 289905-88-0

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.76%

产品类别：: 抑制剂

货号：: T6186

Product Introduction

Bioactivity

Name	TRAM-34
Description	TRAM-34 (Triarylmethane-34) (Kd=20 nM), an effective and specific inhibitor of the intermediate-conductance Ca2+-activated K+ channel (IKCa1, KCa3.1), does not block cytochrome P450. The selective activity of TRAM-34(TRAM 34) is 200 to 1500-fold than other ion channels.
Cell Research	Cells are exposed to TRAM-34 for 48 hours. After 48 hours, cells are harvested by suction (suspension cells) or by trypsinization (adherent cell lines), centrifuged, resuspended in 0.5 mL PBS containing 1 μg/mL propidium iodide (PI), and red fluorescence measured on a FACScan flow cytometer. The percentage of dead cells is determined by their PI uptake, 104 cells of every sample being analyzed. (Only for Reference)
Kinase Assay	Electrophysiology: The human IKCa1 is cloned and expressed in COS-7 cells. Cells are studied in the whole-cell configuration of the patch-clamp technique. The holding potential is 280 mV. The internal pipette solution contains: 145 mM K+ aspartate, 2 mM MgCl2, 10 mM Hepes, 10 mM K2EGTA, and 8.5 mM CaCl2 (1 μM free Ca2+), pH 7.2, 290-310 mOsm. To reduce currents from the native chloride channels in COS-7 cells, Na+ aspartate Ringer is used as an external solution: 160 mM Na+ aspartatey/4.5 mM KCl/2 mM CaCl2/1 mM MgCl2/5 mM Hepes, pH 7.4/290-310 mOsm. IKCa currents in COS-7 cells are elicited by 200-ms voltage ramps from -120 mV to 40 mV applied every 10 seconds and the reduction of slope conductance at -80 mV by TRAM-34 taken as a measure of channel block.
In vitro	与克霉唑不同，TRAM-34选择性地抑制IKCa1，而不阻断细胞色素P450酶(CYP3A4)。TRAM-34在IKCa1转染的COS-7细胞中以及人类T淋巴细胞和T84细胞中的天然IKCa电流上，比克霉唑更强效地抑制克隆IKCa1通道。TRAM-34展现出对其他离子通道，如KV、BKCa、SKCa、Na+、CRAC和Cl-通道。TRAM-34显著地以295-910 nM和85-830 nM的IC50，分别抑制抗CD3 Ab或PKC激活剂PMA加钙离子载体离子霉素诱导的人类T淋巴细胞激活。5 μM的TRAM-34不影响人类T淋巴细胞或几种细胞线的细胞活力。[1] TRAM-34显著抑制EGF诱导的IKCa1上调，并以8 nM的IC50，抑制A7r5细胞的EGF刺激的增殖。[2] TRAM-34处理抑制人类子宫内膜癌(EC)细胞的增殖，并阻断EC细胞周期在G0/G1期。[3]
In vivo	TRAM-34以约500-1,000倍于阻断剂量（0.5 mg/kg/day）的剂量连续治疗7天，对小鼠无毒性。[1] 以120 mg/kg/day的剂量给予TRAM-34能显著减少约40%的大鼠气球导管损伤（BCI）模型中的内膜增生。[2] 与其体外抑制EC细胞增殖的作用一致，30 μM的TRAM-34治疗能够减缓HEC-1-A肿瘤在体内的发展。[3]
Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
Solubility Information	DMSO : 3.5 mg/mL (10 mM), Heating is recommended.
Keywords	TRAM-34 \| Inhibitor \| Potassium Channel \| KcsA \| inhibit \| TRAM34
Inhibitors Related	Tetraethylammonium chloride \| Amlexanox \| Cloperastine hydrochloride \| Quinine \| Resveratrol \| 2,2,2-Trichloroethanol \| Minoxidil sulfate \| Tetraethylammonium bromide \| Indapamide \| (±)-Naringenin \| Butamben \| Halothane
Related Compound Libraries	Inhibitor Library \| Anti-Breast Cancer Compound Library \| Bioactive Compound Library \| Bioactive Compounds Library Max \| Potassium Channel Targeted Library \| Anti-Aging Compound Library \| HIF-1 Signaling Pathway Compound Library \| Ion Channel Targeted Library \| Membrane Protein-targeted Compound Library \| Pain-Related Compound Library

Triarylmethane-34|||TRAM 34|TargetMol

TargetMol Chemicals Inc. 总部位于马萨诸塞州波士顿，致力于为全球生化领域科学家的研究提供专业的产品和服务。TargetMol?品牌的客户群分布于40多个国家和地区，已发展成为全球知名的化合物库和小分子化合物研究供应商。 TargetMol?可提供160多种满足不同需求的化合物库，以及多种类型的生化试剂产品，包括12000多种抑制剂、16000多种天然产物和各类多肽、抗体、生命科学试剂盒等，此外，我们还建设有CADD（计算机辅助药物设计）研究中心、药理实验室、药化合成平台三大技术中心，全方位满足客户的定制需求。凭借我们优质的产品和服务、快速高效的全球供应链和专业的技术支持，我们将有效帮助您缩短研发周期，取得更成功的结果。

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