化合物 Tubastatin A|T1966|TargetMol

Tubastatin A
1252003-15-8

￥287 10mg 起订

￥463 25mg 起订

￥672 50mg 起订

上海更新日期：2024-09-29

TargetMol中国（陶术生物）

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联系人：邵小姐

手机：4008200310 拨打

邮箱：marketing@tsbiochem.com

产品详情:

中文名称：: 化合物 Tubastatin A

英文名称：: Tubastatin A

CAS号：: 1252003-15-8

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.51%

产品类别：: 抑制剂

货号：: T1966

Product Introduction

Bioactivity

Name	Tubastatin A
Description	Tubastatin A (Tubastatin A BASE) is an effective and specific HDAC6 inhibitor (IC50: 15 nM, in a cell-free assay). Its selectivity is 1000-fold against all the other isozymes except HDAC8.
Cell Research	Anchorage-independent growth is assessed by growing cells in soft agar. About 25,000 cells suspended in 0.4% agar in culture media are layered over a 1% agar layer in a 6-well plate. Media are added twice a week and pictures are taken after 21 days of incubation. The number and size of colonies are analyzed using the Gel-Pro software.(Only for Reference)
Kinase Assay	HDAC enzymatic assays: Tubastatin A is dissolved and diluted in assay buffer (50 mM HEPES, pH 7.4, 100 mM KCl, 0.001% Tween-20, 0.05% BSA, and 20 μM tris(2-carboxyethyl)phosphine) to 6-fold of the final concentration. HDAC enzymes are diluted to 1.5-fold of the final concentration in assay buffer and pre-incubated with Tubastatin A for 10 minutes before the addition of the substrate. The amount of FTS (HDAC1, HDAC2, HDAC3, and HDAC6) or MAZ-1675 (HDAC4, HDAC5, HDAC7, HDAC8, and HDAC9) used for each enzyme is equal to the Michaelis constant (Km), as determined by a titration curve. FTS or MAZ-1675 is diluted in assay buffer to 6-fold the final concentration with 0.3 μM sequencing grade trypsin. The substrate/trypsin mix is added to the enzyme/compound mix and the plate is shaken for 60 seconds and then placed into a SpectraMax M5 microtiter plate reader. The enzymatic reaction is monitored for release of 7-amino-4-methoxy-coumarin over 30 minutes, after deacetylation of the lysine side chain in the peptide substrate, and the linear rate of the reaction is calculated.
In vitro	与对照组相比,Tubastatin A 可使PCNA阳性细胞的量显著降低(34% vs 65%).在同基因大鼠原位胆管癌模型中,Tubastatin A（10 mg/kg）降低瘤重与肝重和体重（分别为5和5.6倍）的比值.在给弗氏完全佐剂诱导的动物炎症模型中,Tubastat A（30 mg/kg,i.p.）对鼠爪体积有显著抑制作用.Tubastat A（30 mg/kg,i.p.）处理胶原诱导型关节炎DBA1小鼠的爪子组织,使临床评分（70％）和IL-6表达显著降低.
In vivo	Tubastatin A（5 μM）剂量依赖性地使神经元细胞免于同型半胱氨酸诱导的死亡,10 μM时可完全避免。 Tubastatin A（10 μM）对组蛋白乙酰化有轻微诱导作用。作用于胆管癌细胞系时,Tubastatin A（10 μM）促使乙酰化α-tubulin水平增加和初级纤毛表达的恢复,初级纤毛表达的恢复与Hedgehog、MAPK信号通路下调、细胞增殖率（平均50％）和浸润（40％）减少相关。作用于脂多糖刺激的人类 THP-1巨噬细胞时,Tubastatin A明显抑制TNF-α和IL-6（IC50：272/712 nM）。作用于小鼠Raw 264.7巨噬细胞时,Tubastatin A剂量依赖性抑制一氧化氮分泌（IC50：4.2 μM）。
Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
Solubility Information	DMSO : 35 mg/mL (104.35 mM), Sonication is recommended. Ethanol : < 1 mg/mL (insoluble or slightly soluble) H2O : < 1 mg/mL (insoluble or slightly soluble)
Keywords	Autophagy \| Inhibitor \| Apoptosis \| inhibit \| HDAC \| Histone deacetylases \| Tubastatin A
Inhibitors Related	Guanidine hydrochloride \| Naringin \| Taurine \| Gefitinib \| Hydroxychloroquine \| 5-Fluorouracil \| Curcumin \| Stavudine \| Tributyrin \| L-Ascorbic acid \| Paeonol \| Sodium 4-phenylbutyrate
Related Compound Libraries	Inhibitor Library \| Bioactive Compound Library \| Bioactive Compounds Library Max \| Autophagy Compound Library \| Apoptosis Compound Library \| Anti-Aging Compound Library \| Highly Selective Inhibitor Library \| HIF-1 Signaling Pathway Compound Library \| NF-κB Signaling Compound Library \| Reprogramming Compound Library

Tubastatin A BASE|TargetMol

TargetMol Chemicals Inc. 总部位于马萨诸塞州波士顿，致力于为全球生化领域科学家的研究提供专业的产品和服务。TargetMol?品牌的客户群分布于40多个国家和地区，已发展成为全球知名的化合物库和小分子化合物研究供应商。 TargetMol?可提供160多种满足不同需求的化合物库，以及多种类型的生化试剂产品，包括12000多种抑制剂、16000多种天然产物和各类多肽、抗体、生命科学试剂盒等，此外，我们还建设有CADD（计算机辅助药物设计）研究中心、药理实验室、药化合成平台三大技术中心，全方位满足客户的定制需求。凭借我们优质的产品和服务、快速高效的全球供应链和专业的技术支持，我们将有效帮助您缩短研发周期，取得更成功的结果。

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