4-吡啶羧酸 2-苯基酰肼|T1869|TargetMol

PluriSIn 1
91396-88-2

￥289 5mg 起订

￥463 10mg 起订

￥793 25mg 起订

上海更新日期：2024-12-02

TargetMol中国（陶术生物）

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产品详情:

中文名称：: 4-吡啶羧酸 2-苯基酰肼

英文名称：: PluriSIn 1

CAS号：: 91396-88-2

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.59%

产品类别：: 抑制剂

货号：: T1869

Product Introduction

Bioactivity

名称	PluriSIn 1
描述	PluriSIn 1 (NSC 14613) is an inhibitor of stearoyl-CoA desaturase (SCD1), the key enzyme in oleic acid biosynthesis, highlighting a unique role for lipid metabolism in hPSCs.
细胞实验	Relative cell numbers are determined by fixating the cells with 0.5% glutardialdehyde and staining with methylene blue dissolved in 0.1 M boric acid (pH 8.5). Color extraction is performed using 0.1 M hydrochloric acid, and the staining (which is proportional to cell number) is quantitated by measuring absorbance at 650 nM.(Only for Reference)
激酶实验	SCD1 activity assays: Cells are plated in 6-well plates at a density of 50k to 100k cells per well. 24 h later, 20 μM PluriSIn #1 or 0.2% DMSO-control are added to the cells. After 12 h of incubation at 37 ℃, 5% CO2, the old medium is removed, cells are washed with PBS, and new medium containing 2.3 μM of 0.75 UCi [1-14C] Stearic Acid is added. The cells are incubated for up to 4 h at 37 ℃, 5% CO2. After the incubation period, the medium is discarded and the cells are washed 3 times with 2 mL of PBS. 2 mL of the mixture n-hexane: isopropanol (3:2 v:v) are added, and the cells are incubated for 30 min at 37 ℃, 5% CO2. 2 mL Folch solution (chloroform: methanol,2:1,v:v) are subsequently added. The liquid is transferred to tubes for phase partition by adding 1 mL water. The lower organic phase is evaporated and used for lipid saponification and TLC separation of the free [1-14C] Stearic Acid (substrate) and [1-14C] Oleic Acid (formed product). Lipids extracted from the cells are applied to TLC plates previously immersed in 10% NO3 Ag and activated at 120℃x60 min. Unlabeled stearic and oleic acid are added to each application point as carriers and as internal standards for identification. The plates are run with a solvent mixture of Chloroform:MeOH:AcH:DDW (90:8:1:0.8). The free fatty acids are detected by U.V. after spraying the TLC with a 2',7',dichlorofluorescein solution. The spots corresponding to stearic and oleic acid are scraped and the radioactivity counted in a sc intillating counter. SCD1 desaturase activity is calculated from the percent conversion of substrate to product and the conversion to pmol/min/106 cells.
体内活性	PluriSIn 1通过激活细胞凋亡使中央细胞死亡。PluriSIn 1通过与hPSCs的作用产生ER应激。PluriSIns 1（20 μM）对未分化hPSCs形成畸胎瘤的过程具有抑制作用,其还抑制mPSCs和小鼠胚胎发育。PluriSIns 1（20 μM）通过与hPSCs作用诱导蛋白质合成降低约30%。作用于hPSCs时,PluriSIns 1可产生强效快速且具有选择性的细胞毒性,还可诱导硬脂酰辅酶A脱氢酶活性降低~65%。
存储条件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	DMSO : 50 mg/mL (234.48 mM), Sonication is recommended. Ethanol : 21.3 mg/mL (100 mM)
关键字	Apoptosis \| Stearoyl-CoA Desaturase (SCD) \| PluriSIn-1 \| NSC14613 \| Inhibitor \| PluriSIn1 \| inhibit \| PluriSIn 1 \| NSC-14613
相关产品	L-Glutamic acid \| Metronidazole \| 5-Fluorouracil \| Dextran sulfate sodium salt (MW 4500-5500) \| Stavudine \| Tributyrin \| Myricetin \| Sorafenib \| L-Ascorbic acid \| Acetylcysteine \| Salicylic acid \| Sodium 4-phenylbutyrate
相关库	抑制剂库 \| 脂代谢化合物库 \| 经典已知活性库 \| 已知活性化合物库 \| 代谢化合物库 \| 细胞凋亡化合物库 \| 抗衰老化合物库

PluriSln 1|||PluriSIn #1|||NSC 14613|||4-吡啶羧酸 2-苯基酰肼|TargetMol

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期	(12年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
主营行业	化学试剂,生物活性小分子