木香烃内酯|T2902|TargetMol

Costunolide
553-21-9

￥179 1mg 起订

￥387 5mg 起订

￥555 10mg 起订

上海更新日期：2024-09-24

TargetMol中国（陶术生物）

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邮箱：marketing@targetmol.com

产品详情:

中文名称：: 木香烃内酯

英文名称：: Costunolide

CAS号：: 553-21-9

品牌：: TargetMol

产地：: 美国

保存条件：: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 91.75%

产品类别：: 抑制剂

货号：: T2902

Product Introduction

Bioactivity

名称	Costunolide
描述	Costunolide (Costus lactone) has anti-inflammatory and anti-oxidant properties and mediates apoptosis.
细胞实验	1) Plate 500-10,000 cells in 200 μL media per well in a 96 well plate. Leave 8 wells empty for blank controls. 2) Incubate (37 °C, 5% CO2) overnight to allow the cells to attach to the wells. 3) Add 2 μL of Costunolide dissolved in DMSO to each well. Place on a shaking table, 150 rpm for 5 minutes, to thoroughly mix the samples into the media. 5) Incubate (37 °C, 5% CO2) for 48 hours to allow Costunolide to take effect. 6) Make 2 mL or more of MTT solution per 96 well plate at 5 mg/mL in PBS. Do not make a stock as MTT in solution is not stable long-term. 7) Add 20 μL MTT solution to each well. Place on a shaking table, 150 rpm for 5 minutes, to thoroughly mix the MTT into the media. 8) Incubate (37 °C, 5% CO2) for 1-5 hours to allow the MTT to be metabolized. 9) Dump off the media. (Dry plate on paper towels to remove residue if necessary. 10) Resuspend formazan (MTT metabolic product) in 200 μL DMSO. Place on a shaking table, 150 rpm for 5 minutes, to thoroughly mix the formazan into the solvent. 11) Read optical density at 560 nm and subtract background at 670 nm. Optical density should be directly correlated with cell quantity. (Only for Reference)
激酶实验	Telomerase activity assay: The telomerase activity is measured by the TRAP assay using the TRAPez Telomerase Detection Kit, which includes primers of a 36-bp internal control (IC) for quantifying the amplification of telomerase activity within a linear range close to 2.5 logs. For RNase treatment, 10μL of extract are incubated with 1μg of RNase at 37 °C for 20 minutes. The products of the TRAP assay are resolved by electrophoresis in a nondenaturing12% PAGE in a buffer containing 0.5 × Tris–borate EDTA and detected by autoradiograph. For quantification of TRAP products, the dried gels are exposed to Fuji Imaging Plate at room temperature. Results are corrected for background, and a standard value of 100 is given to the untreated control cell signal. Signal intensities of Costunolide-treated cells are compared to the standard and are expressed as a fraction of the maximum value of 100. [1]
体外活性	Costunolide可降低小鼠角膜微囊模型中由乙烯基酯树脂鳞片胶泥诱导的新生血管形成.Costunolide通过阻断血管生长因子信号转导通路来抑制血管生成.
体内活性	Costunolide连续处理人肿瘤细胞(A549,SK-OV-3,SK-MEL-2,XF498和HCT-1)48小时,可以剂量依赖性抑制细胞增殖。Costunolide通过ROS介导的线粒体通透性转换和细胞色素C在HL-60人类白血病细胞中释放细胞溶质来诱导细胞凋亡。Costunolide具有明显的抗真菌活性,包括毛癣菌、猴毛癣菌,红色毛癣菌等。Costunolide抑制MCF-7和MDA-MB-231端粒酶活性和细胞增殖,该抑制作用呈浓度和时间依赖性。Costunolide也剂量依赖性抑制由法尼基蛋白转移酶诱导的人核纤层蛋白B法尼基化过程。
存储条件	store at low temperature \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	DMSO : 45 mg/mL (193.7 mM) Ethanol : 11.6 mg/mL (50 mM)
关键字	Inhibitor \| Apoptosis \| antioxidants \| anti-allergic \| regenerating \| anticancer \| antimicrobial \| antidiabetic \| Costunolide \| anti-inflammatory \| Endogenous Metabolite \| growth \| bone \| NSC106404 \| neuroprotective \| NSC-106404 \| promoting \| inhibit \| hair
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木香烃内酯|||Costus lactone|||NSC 106404|||Costunolid|||(+)-Costunolide|TargetMol

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期	(12年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
主营行业	化学试剂,生物活性小分子