补骨脂二氢黄酮|T3846|TargetMol

Bavachin
19879-32-4

￥143 5mg 起订

￥188 10mg 起订

￥289 25mg 起订

上海更新日期：2024-09-24

TargetMol中国（陶术生物）

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联系人：邵小姐

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邮箱：marketing@targetmol.com

产品详情:

中文名称：: 补骨脂二氢黄酮

英文名称：: Bavachin

CAS号：: 19879-32-4

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 100%

产品类别：: 抑制剂

货号：: T3846

Product Introduction

Bioactivity

Name	Bavachin
Description	Bavachin (Corylifolin) is a phytoestrogen that activates the estrogen receptors ERα and ERβ.
Cell Research	Bavachin is dissolved in DMSO. MTT solution (20 μL) is added to each well of the 96-well plates, the cells are cultured for 4 h, the solution is discarded, and the purple crystal is dissolved in the wells with 150 μL DMSO solution, agitated in a 37°C incubator shaker for 10 min, and the optical density (OD) is measured at 490 nm by the microplate reader.
Kinase Assay	The chemiluminescent assay is used to confirm PCSEE MAO-A and MAO-B inhibitory effects and to test BNN and BVN hMAO-A and hMAO-B inhibition using MAO-Glo kit. Each enzyme's Arbitrary Light Unit (ALU) is measured in the presence of PCSEE, BNN, BVN, and standard DEP as an MAO-BI positive control. Briefly, hMAO-A and hMAO-B isozymes are diluted to 2× with reaction buffer (pH 7.4) and preincubated with 4× PCSEE, BNN, BVN, or DEP working solutions at RT for 30?min in white opaque 96-well plates. For determining activity inhibition, final 8.5?μg/mL concentrations of PCSEE, BNN, BVN, and DEP are used. For IC50 determination, 8× PCSEE and BNN working solutions are serially diluted using reaction buffers (pH 7.4) to make a 4× concentration. Ten points' range of PCSEE (1.0 to 250.0?μg/mL) and BNN (up to 400?μM (135.4?μg/mL)) final concentrations is used. Controls used are with and without ethanol. Ethanol solvent in controls is kept to a maximum final (volume) of ≤2%. Each isozyme is substituted with the reaction buffer for the blank. Based on our preliminary optimizations and Valley's method, the reaction is initiated by adding 4× luciferin derivative substrate (LDS) for a final (concentration) of 40 and 4?μM for hMAO-A and hMAO-B reactions, respectively. The final volume per well of each reaction is 50?μL. The reaction is optimized for the amount of A and B enzyme used to be incubated for less than 3.5?h at RT. To stop the reaction and produce the luminescence signal RLDR is added to all wells, 50?μL to each well, and incubated for a further 30?min.
In vitro	Bavachin显著抑制黑色素合成和TYR活性。在10 μM浓度下，Bavachin抑制A375细胞中TYR和JNK蛋白的表达，以及TYR, TRP-1, TRP-2, ERK1, ERK2和JNK2 mRNA的表达。ICI182780和U0126能显著逆转Bavachin对这些蛋白和mRNA表达水平的影响[1]。在ORO染色实验中，Bavachin以剂量依赖的方式积累脂质。与对照细胞相比，10 μM Bavachin显著增加前脂肪细胞的生长，这一结果通过MTT实验确认。Bavachin还增加了前脂肪细胞增殖过程中新合成DNA中BrdU的掺入，胰岛素可以增强BrdU的掺入，而2和10 μM Bavachin与胰岛素共处理效果更佳。Bavachin激活脂肪生成因子并增加分化脂肪细胞中PPARγ的转录活性。通过Akt和AMPK途径，Bavachin增强胰岛素刺激的GLUT4介导的葡萄糖摄取[2]。BVN显著增加hMAO-A和hMAO-B活性[3]。Bavachin在与重组ER的[3H] E2竞争位点结合实验中表现出ER配体结合活性。通过使用ERα或ERβ和雌激素反应性荧光素酶质粒在CV-1细胞中的瞬时转染体系，测定了Bavachin的雌激素活性，分别为EC50 320 nM和680 nM。Bavachin增加雌激素反应性基因如pS2和PR的mRNA水平，并通过蛋白酶体途径降低ERα的蛋白水平[4]。
Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
Solubility Information	DMSO : 58 mg/mL (178.8 mM) Chloroform, Dichloromethane, Ethyl Acetate : Soluble
Keywords	Bavachin \| Estrogen Receptor/ERR \| Inhibitor \| inhibit
Inhibitors Related	Astragaloside IV \| Natamycin \| Estradiol \| Melatonin \| Mifepristone \| Estradiol benzoate \| Ethisterone \| Tamoxifen \| Allura Red AC \| Kaempferol \| Cholesterol \| Chrysin
Related Compound Libraries	Bioactive Compound Library \| Selected Plant-Sourced Compound Library \| Bioactive Compounds Library Max \| Flavonoid Natural Product Library \| Anti-Tumor Natural Product Library \| Natural Product Library \| Traditional Chinese Medicine Monomer Library \| Nuclear Receptor Compound Library \| Natural Product Library for HTS

补骨脂甲素|||补骨脂二氢黄酮|||Corylifolin|TargetMol

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期	(12年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
主营行业	化学试剂,生物活性小分子