小鼠ADAM17蛋白-ACROBiosystems百普赛斯

Mouse ADAM17 / TACE / CD156b Protein, His Tag

分子别名（Synonym）

ADAM17,TACE,CD156b

表达区间及表达系统（Source）

Mouse ADAM17, His Tag (AD7-M52H1) is expressed from human 293 cells (HEK293). It contains AA Pro 18 - Asp 563 (Accession # Q9Z0F8).

Predicted N-terminus: Pro 18, Asp 59 and Arg 215

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 63.4 kDa. The protein migrates as 19 kDa and 20 kDa (Propeptide), 55-66 kDa (Mature-ADAM17) and 75-94 kDa (Pro-ADAM17) under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM MES, 150 mM NaCl, pH6.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

含去整合素和金属蛋白酶结构域的蛋白17（ADAM17）是去整合素和金蛋白酶ADAM蛋白家族的成员，也称为TNF-α转化酶、TNF-α转换酶和CD156b，CD156b含有一个去整合素结构域和一个肽酶M12B结构域。ADAM17可以将TNFα的膜结合前体切割成其成熟的可溶性形式。ADAM17还负责可溶性JAM3从内皮细胞表面的蛋白水解释放（通过相似性）和其他几种细胞表面蛋白的蛋白水解分泌，包括p75 TNF受体、白细胞介素1受体II型、p55 TNF受体、转化生长因子α、L-选择素、生长激素受体、MUC1和淀粉样前体蛋白。此外，ADAM17通过介导Notch的切割，产生称为Notch细胞外截短的膜相关中间片段，从而充当Notch通路的激活剂。