小鼠VEGFA蛋白-ACROBiosystems百普赛斯

Mouse VEGFA Protein, Tag Free (HPLC-verified)

分子别名（Synonym）

RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF

表达区间及表达系统（Source）

Mouse VEGFA Protein, Tag Free (VE4-M4216) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 190 (Accession # AAB22253.1 ).

Predicted N-terminus: Ala 27

蛋白结构（Molecular Characterization）

This protein carries no "tag".

The protein has a calculated MW of 19.3 kDa. The protein migrates as 23-30 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

无菌（Sterility）

Negative

纯度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-HPLC.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

血管内皮生长因子A（VEGFA）也称为血管通透性因子（VPF）。VEGFA属于PDGF/VEGF生长因子家族。VEGFA是一种糖基化丝裂原，特异性作用于内皮细胞，具有多种作用，包括介导血管通透性增加、诱导血管生成、血管生成和内皮细胞生长、促进细胞迁移和抑制凋亡。或者，编码自由分泌或细胞相关异构体的剪接转录变体已被表征。由于选择性剪接，VEGFA由一组三种主要亚型产生，如果产生任何三种亚型（VEGFA120、VEGFA164和VEGFA188），则不会导致小鼠血管缺陷和完全VEGFA敲除死亡。