小鼠IL-2蛋白-ACROBiosystems百普赛斯

Mouse IL-2 Protein, His Tag

分子别名（Synonym）

IL2,TCGF,lymphokine,Interleukin 2

表达区间及表达系统（Source）

Mouse IL-2, His Tag (IL2-M52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Gln 169 (Accession # P04351-1).

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 19.1 kDa. The protein migrates as 22-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白细胞介素-2（IL-2）是一种白介素，一种细胞因子免疫系统信号分子，是一种促白细胞激素，在身体对微生物感染的自然反应中起着重要作用，并有助于区分外来（非自身）和自身。IL-2通过与淋巴细胞（负责免疫的细胞）表达的IL-2受体结合来介导其作用。成熟的人IL-2与小鼠和大鼠IL-2的aa序列同源性分别为56%和66%。人和小鼠IL-2具有跨物种活性。IL-2受体由三个亚基组成，它们以不同的预形成复合物存在于细胞表面。IL-2在胸腺T细胞发育过程中也是必要的，用于成熟被称为调节性T细胞（T-reg）的独特T细胞亚群。从胸腺中排出后，T-Regs的功能是防止其他T细胞识别和对抗“自身抗原”，这可能会导致“自身免疫”。T-Regs通过阻止反应细胞产生IL-2来实现这一目的。因此，需要IL-2来区分自我和非自我，这是免疫系统的另一个独特特征。