长链重组人胰岛素样生长因子1Human Long Arg3 Insulin-like Growth Factor-I(Long-R3-IGF-I)

General Information：

Human Long Arg3 Insulin-like Growth Factor-I(Long-R3-IGF-I) is an 83 amino acid analog of human IGF-I comprising the complete human IGF-I sequence with the substitution of an Arg for the Glu at position 3 (hence R3), and a 13 amino acid extension peptide at the N-terminus. Scientists have engineered this analog with the express purpose of increasing biological activity.

Human Long-R3-IGF-I is significantly more potent than human IGF-I in vitro. The enhanced potency is due to the markedly decreased binding of human Long-R3-IGF-I to IGF binding proteins which normally inhibit the biological actions of IGFs.

Expression Host: E. coli

Molecular Mass:

The recombinant human Long-R3-IGF-I has a calculated molecular mass of 9111Da.

Formulation:

Lyophilized from sterile PBS , pH 7.2, and 5 % mannitol are added as protectants before lyophilization.

Quality Control

Purity: > 95 % as as determined by the following methods:

(a) RP-HPLC analysis

(b) SDS-PAGE silver-stained gel analysis

Bio-activity:

The ED50 of recombinant human Long R3 IGF-I, calculated by the dose-dependant proliferation of murine BALBC 3T3 cells, is less

than 1.0ng/ml, corresponding to a specific activity of 1.0×106IU/mg.

Endotoxin:

﹤ 1.0 EU/mg as determined by the LAL method. Product specification：

100ug/vial；500ug/vial；1mg/vial.

Store at：

2-8oC for two years，Avoid repeated freeze and thaw.