IBMX,3-异丁基-1-甲基黄嘌呤|T1713|TargetMol

IBMX
28822-58-4

￥145 5mg 起订

￥192 10mg 起订

￥293 25mg 起订

上海更新日期：2024-09-23

TargetMol中国（陶术生物）

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产品详情:

中文名称：: IBMX,3-异丁基-1-甲基黄嘌呤

英文名称：: IBMX

CAS号：: 28822-58-4

品牌：: TargetMol

产地：: 美国

保存条件：: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.86%

产品类别：: 抑制剂

货号：: T1713

Product Introduction

Bioactivity

Name	IBMX
Description	IBMX (Methylisobutylxanthine) is a broad-spectrum phosphodiesterase (PDE) inhibitor with inhibitory activity against PDE3, PDE4, and PDE5 (IC50=6.5/26.3/31.7 μM). IBMX enhances the intracellular cAMP level.
Cell Research	Intracellular cyclic GMP and cyclic AMP concentrations in guinea-pig TSMCs were assayed as previously described. In brief, cells were grown in 24-well plates 10^5 cells per well. At confluence, monolayer cells were washed with phosphate buffer solution (PBS) and then incubated with KMUP-1 (0.1–100 μM) in the presence of 100 μM IBMX for 20 min. Incubation was terminated by the addition of 10% trichloroacetic acid (TCA). Cell suspensions were sonicated and then centrifuged at 2500 × g for 15 min at 4°C. To remove TCA, the supernatants were extracted three times with 5 volumes of water-saturated diethyl ether. Then, the supernatants were lyophilized and the cyclic GMP or AMP of each sample was determined by using commercially available radioimmunoassay kits [1].
Animal Research	Male mice (25-35 g), obtained from the animal house of Faculty of Medicine, were kept in controlled environmental conditions (temperature: 23±2 oC; light-dark cycle: 7 a.m. to 7 p.m.) and were divided randomly into groups of seven. All test compounds were dissolved in DMSO and diluted to desired concentration with less than 1% DMSO. For the experiment, the test compound (IBMX, milrinone, MCPIP, mc1, mc2, mc5 or mc6) or solvent (control) was injected subcutaneously to mice at 1 mg/kg dosage twice a day (8:00 a.m. and 8:00 p.m.) for 7 days. On day 8, animals were anesthetized with intraperitoneal injection of thiopental (80 mg/kg) and blood samples were obtained from their hearts and then the liver was dissected. Each sample was centrifuged for 5 min and its serum was separated [3].
In vitro	方法：从 HK 喂养的大鼠分离的 CCD 用 IBMX (100 μM) 预处理 20 min，并检查 ANG II 或 cGMP 对通道活性的影响。结果：IBMX 激活了 ROMK 通道并阻止了 ANG II 进一步激活通道。[1] 方法：豚鼠 TSMC 的原代培养物，在 IBMX (100 μM) 存在的情况下，检测 KMUP-1 对 cAMP 和 cGMP 水平的影响。结果：IBMX 和 KMUP-1 显著增加了 cAMP 和 cGMP 水平。单独使用 KMUP-1 对 cAMP 和 cGMP 水平与在有 IBMX 的条件下没有显著差异。[2] 方法：哺乳动物细胞 CHO 用 IBMX (10-1000 μM) 处理，使用膜片钳技术测量全细胞电流。结果：IBMX 对 THIK-1 电流影响的稳态剂量反应曲线可以拟合为 Hill 系数为 1 和 IC50 为 120 μM。[3]
In vivo	方法：为检测对小鼠的代谢作用，将 IBMX (1 mg/kg) 皮下注射给小鼠，每天两次，持续七天。结果：IBMX 显著增加了小鼠的血糖水平（血糖，mg/dl，对照=141，IBMX=210）。[4] 方法：为检测对高血糖小鼠的代谢作用，将葡萄糖 (0.5 g/kg) 和 IBMX (1 mg/kg) 股静脉注射给 Wistar 大鼠。结果：在高血糖大鼠中，IBMX 能降低血糖，IBMX 不会改变血浆胰岛素水平，IBMX 降低了肝糖原储存。[4]
Storage	store at low temperature \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
Solubility Information	H2O : 2.22 mg/mL (10 mM), Sonication is recommended. DMSO : 16.7 mg/mL (75 mM) 10% DMSO+90% Saline : 1.67 mg/mL (7.51 mM), Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. Ethanol : 22.24 mg/mL (100 mM), Sonication is recommended.
Keywords	Phosphodiesterase (PDE) \| IBMX \| inhibit \| Inhibitor
Inhibitors Related	Icariin \| Vardenafil hydrochloride \| Apremilast \| Indomethacin \| Roflumilast \| Theophylline \| Theobromine \| Theophylline monohydrate \| Isoprenaline hydrochloride \| Doxofylline
Related Compound Libraries	Inhibitor Library \| Bioactive Compound Library \| Bioactive Compounds Library Max \| Bioactive Lipid Compound Library \| Metabolism Compound Library \| Anti-Alzheimer's Disease Compound Library \| Anti-Cardiovascular Disease Compound Library \| Anti-Neurodegenerative Disease Compound Library \| Anti-Metabolism Disease Compound Library \| Human Metabolite Library

Methylisobutylxanthine|||Isobutylmethylxanthine|||IBMX,3-异丁基-1-甲基黄嘌呤|||3-Isobutyl-1-methylxanthine|||1-Methyl-3-Isobutylxanthine|TargetMol

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成立日期	(12年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
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