化合物 Thiazovivin|T2155|TargetMol

Thiazovivin
1226056-71-8

￥207 1mg 起订

￥373 2mg 起订

￥663 5mg 起订

上海更新日期：2024-09-23

TargetMol中国（陶术生物）

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产品详情:

中文名称：: 化合物 Thiazovivin

英文名称：: Thiazovivin

CAS号：: 1226056-71-8

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 98.75%

产品类别：: 抑制剂

货号：: T2155

Product Introduction

Bioactivity

Name	Thiazovivin
Description	Thiazovivin, a ROCK inhibitor (IC50: 0.5 μM), increases the survival rate of hESC.
Cell Research	Chemically defined and feeder-free human embryonic stem cell (hESC) culture was described briefly as following. hESCs were grown on Matrigel-coated tissue culture plates in N2B27-CDM [DMEM-F12 supplemented with 1× N2 supplements, 1× B27 supplements, 2 mM L glutamine, 0.11 mM 2-mercaptoethanol, 1× nonessential amino acids, and 0.5 mg/mL BSA (fraction V)] and 20 ng/mL bFGF. Human ESCs were passaged every five to six days with 0.05% trypsin. Murine ESCs are cultured in knockout DMEM supplement with 2 mM L glutamine, 1× nonessential amino acids, 15% serum replacement, and 1 × 10^3 ng/mL leukemia inhibitory factor (LIF). For clonal survival assays, single hESCs were diluted to clonal density and plated onto 96-well Matrigel-coated plate. For low-density survival assays, 500 cells were plated onto 96-well Matrigel-coated plate. To visualize hESC colonies, cultures were fixed in 4% paraformaldehyde in phosphate buffered saline (PBS) for 5 min, washed once in PBS, then stained for alkaline phosphatase (ALP) activity as described in the manufacturer's instructions. ALP-positive colonies were counted on an inverted microscope. For growing hESCs in mouse medium, HES2, HUES7, HUES9, and HUES1-Oct4-GFP were cultured in murine ESC (mESC) growth media supplemented with 1-μM mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK) inhibitor PD0325901 and 5-μM p38 inhibitor SB202190 and 1 × 10^3 human LIF [1].
Kinase Assay	Synthesis of compound immobilized affinity matrixes: Compounds Tzv and its inactive analog (10 mg each) in DMSO (500 μL) and Et3N (10.4 μL) were added to Reacti-Gel (0.5 mL, 25 μmol) that was washed by DMSO in an Eppendorf vial. The reaction mixture was incubated at room temperature until the starting material disappeared (determined by HPLC). After the disappearance of the starting material, ethanolamine (15 μL) was added and the resulting mixture was incubated at room temperature overnight to block the Reacti-Gel. The resulting affinity matrices were washed thoroughly with DMSO (500 μL × 4), PBS (500 μL × 2), and stored at 4 °C in NaN3 solution (0.1% in PBS). Affinity pull-down was performed as described previously. Briefly, whole cell lysates were pretreated with the unfunctionalized affinity matrix at 4 °C for 1.5 h. After washing three times, samples were incubated with the positive or negative affinity matrix at 4 °C for 1 h. After heat shock, samples were loaded and separated on a 4–20% Tris-Glycine SDS PAGE and silver stained with a Silver Stain Plus Kit. The differentially retained protein bands were cut, destained, and analyzed with LCMS [1].
In vitro	当细胞在E-cadherin阻断抗体的存在下培养时，细胞在Thiazovivin处理后形成的大型、紧密的聚集体严重受阻，且观察到广泛的细胞死亡。Thiazovivin（2 μM）抑制ROCK活性，并在与Y-27632（10 μM）相似的水平上保护hESCs，后者是一种广泛使用的选择性ROCK抑制剂[1]。在Thiazovivin处理组中，囊胚和胚胎细胞团在给养细胞上的附着率高于对照组。Thiazovivin处理增加了OCT4和NANOG基因的多能性标记以及粘附分子E-cadherin[2]。通过在重编程培养中添加Thiazovivin，CB细胞的重编程效率增加了10倍以上[3]。
Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
Solubility Information	DMSO : 60 mg/mL (192.7 mM)
Keywords	Inhibitor \| Rho-associated protein kinase \| ROK \| Rho-associated kinase \| ROCK \| Thiazovivin \| inhibit \| Rho-kinase
Inhibitors Related	Y-27632 dihydrochloride \| CID-5056270 \| ROCK-IN-5 \| Fasudil hydrochloride \| SB-747651A Dihydrochloride \| Belumosudil \| Tofacitinib \| CAY10746 \| Y-27632 \| RKI-1447 \| SAR407899 hydrochloride \| GSK180736A
Related Compound Libraries	Inhibitor Library \| Bioactive Compound Library \| Anti-Cancer Compound Library \| Bioactive Compounds Library Max \| Kinase Inhibitor Library \| Neural Regeneration Compound Library \| Cytoskeletal Signaling Pathway Compound Library \| NO PAINS Compound Library \| TGF-beta/Smad Compound Library \| Target-Focused Phenotypic Screening Library

Thiazovivin|TargetMol

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期	(12年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
主营行业	化学试剂,生物活性小分子