(R)-2-羟基戊二酸二钠盐|T6820|TargetMol

D-α-Hydroxyglutaric acid disodium
103404-90-6

￥233 5mg 起订

￥323 10mg 起订

￥578 25mg 起订

上海更新日期：2024-09-23

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产品详情:

中文名称：: (R)-2-羟基戊二酸二钠盐

英文名称：: D-α-Hydroxyglutaric acid disodium

CAS号：: 103404-90-6

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 98%

产品类别：: 抑制剂

货号：: T6820

Product Introduction

Bioactivity

Name	D-α-Hydroxyglutaric acid disodium
Description	D-α-Hydroxyglutaric acid disodium (Disodium (R)-2-Hydroxyglutarate) is a competitive inhibitor of α-ketoglutarate-dependent dioxygenases with Ki of 0.628 mM.
Cell Research	Cells are seeded in 12-well plates and, after overnight incubation, treated with the indicated concentrations of each compound. After harvesting, cells are stained with acridine orange (AO) and 4′,6-diamidino-2-phenylindole (DAPI). Cell number and viability are measured based on AO and DAPI fluorescence as measured by NC3000 following the manufacturer's instructions.(Only for Reference)
Kinase Assay	Enzymatic Assays: To assay human JHDM1A/KDM2A demethylase activity toward H3K36me2, His tagged JHDM1A is first obtained by transforming pET28a-JHDM1A into Escherichia coli BL21 and protein expression is induced by addition of 1 mM IPTG at 30° C when cell density reaches 0.5 OD600 units. Cells are lysed by sonication and Ni-NTA agarose is used to purify His-JHDM1A fusion proteins. Histone demethylase assay is carried out by incubating 2 μg oligonucleosomes, 4 μg purified His-JHDM1A, and/or 10–50 mM L- or D-2-HG in histone demethylation buffer [50 mM HEPES (pH 8.0), 625 μM Fe(NH4)2(SO4)2, 0.1–0.5 mM α-KG, 2 mM ascorbate] at 37° C for 2–3 hr and the reactions are stopped by the addition of SDS loading buffer and subsequently analyzed by western blotting using anti-H3K36me2 antibody. To measure CeKDM7A demethylase activity toward H3K9me2 and H3K27me2, two synthetic dimethylated peptides H3K9me2 [ARTKQTARK (me2)STGGKA] and H3K27me2 [QLATKAARK (me2)SAPAS] are used as substrates. Demethylase assays are carried out in the presence of 10 μg enzyme, 1 μg peptide in 20 μl buffer 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 50 μM Fe(NH4)2(SO4)22, 100 μM α-KG, 2 mM Vc, 10 mM PMSF for 3 hr. The demethylation reaction mixture is desalted by passing through a C18 ZipTip. To examine the inhibitory effect of 2-HG, various concentrations of 2-HG are incubated with KDM7A briefly before adding other reaction mixtures. The samples are analyzed by a MALDI-TOF/TOF mass spectrometer.
In vitro	在U-87 mg细胞中，D-α-Hydroxyglutaric acid disodium 作为α-KG的弱拮抗剂，通过抑制α-KG依赖的组蛋白去甲基酶，增加了H3K9和H3K79的双甲基化。[1]此外，D-α-Hydroxyglutaric acid disodium还抑制了ATP合酶和mTOR信号传导，从而导致生长停滞和肿瘤细胞死亡。[2]
Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
Solubility Information	DMSO : < 1 mg/mL (insoluble）
Keywords	DαHydroxyglutaric acid disodium \| ATP Synthase \| excitotoxic \| Endogenous Metabolite \| Mammalian target of Rapamycin \| D-a-Hydroxyglutaric acid disodium \| 5-methlycytosine \| H3K9me2 \| H3K27me2 \| inhibit \| Inhibitor \| mTOR \| IDH2 \| neurotoxic \| IDH1 \| D-α-Hydroxyglutaric acid disodium \| Reactive Oxygen Species \| α-ketoglutarate \| competitor \| D-alpha-Hydroxyglutaric acid \| D-α-Hydroxyglutaric acid \| D-alpha-Hydroxyglutaric acid disodium \| Disodium 2-Hydroxyglutarate \| CeKDM7A \| D α Hydroxyglutaric acid disodium \| D-a-Hydroxyglutaric acid
Inhibitors Related	Glycerol \| Sucrose \| Ferulic Acid \| Thymidine
Related Compound Libraries	Inhibitor Library \| Bioactive Compound Library \| Bioactive Compounds Library Max \| Anti-Pancreatic Cancer Compound Library \| Kinase Inhibitor Library \| Anti-Aging Compound Library \| Ion Channel Targeted Library \| Membrane Protein-targeted Compound Library

Disodium (R)-2-Hydroxyglutarate|||D-alpha-Hydroxyglutaric acid disodium salt|||(R)-2-羟基戊二酸二钠盐|TargetMol

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