瑞香素|T2851|TargetMol

Daphnetin
486-35-1

￥197 5mg 起订

￥328 10mg 起订

￥673 25mg 起订

上海更新日期：2024-09-23

TargetMol中国（陶术生物）

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联系人：邵小姐

电话：021-021-33632979拨打

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邮箱：marketing@targetmol.com

产品详情:

中文名称：: 瑞香素

英文名称：: Daphnetin

CAS号：: 486-35-1

品牌：: TargetMol

产地：: 美国

保存条件：: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.82%

产品类别：: 抑制剂

货号：: T2851

Product Introduction

Bioactivity

Name	Daphnetin
Description	Daphnetin (7,8-Dihydroxycoumarin), a natural coumarin derivative, is a protein kinase inhibitor with inhibitory for EGFR (IC50: 7.67 μM), PKA (IC50: 9.33 μM), and PKC (IC50: 25.01 μM), also exhibit anti-oxidant and anti-inflammatory activities.
Cell Research	The cytostatic effect of Daphnetin tested on the MCF-7 tumor cells is estimated using the microculture MTT assay. The assay is based on the reduction of soluble tetrazolium salt by mitochondria of viable cells. The reduced product, an insoluble purple-colored formazan, is dissolved in dimethyl sulfoxide and measured spectrophotometrically (570 nm). The amount of formazan formed is proportional to the number of viable cells. Cells (3 × 103) are seeded in each of the 96 microplate wells in a 200 μL medium containing the corresponding concentration of daphnetin. Daphnetin is tested at five concentrations (12.5 μM, 25 μM, 50 μM, 100 μM, 200 μM). After 24-hour, 48-hour, and 72-hour exposure, the percentage of proliferative inhibition of treated cells is estimated against the solvent-treated control cells (PI% = [(T/C) ? 1] × 100). PI = proliferation inhibition; T = treated, C = control. IC50 is calculated from the least square concentration-response regressions.(Only for Reference)
Kinase Assay	Assay of protein kinase C (PKC) and cAMP-dependent protein kinase (PKA) activities.: PKC and PKA activities are detected. Briefly, 5 mL of PKC or PKA is mixed with 5 mL of lipid preparation containing 100 mM phorbol 12-myristate 13-acetate, 2.8 mg/mL phosphatidyl serine, and Triton X-100 mixed micelles for PKC assay or 5 mL of 40 mM cAMP in 50 mM Tris-HCl, pH 7.5, for PKA assay, and 5 mL of daphnetin. The reaction is started by adding 10 mL of PKC substrate solution containing 250 mM acetylated myelin basic protein, 100 mM ATP, 5 mM CaCl2, 10 mM MgCl2, 20 mM Tris-HCl, pH 7.5, or 10 mL of PKA substrate solution containing 200 mM Kemptide, 400 mM ATP, 40 mM MgCl2, 1 mg/mL BSA, 50 mM Tris-HCl, pH 7.5, and 20-25 mCi/mL[g-32P] ATP. After incubation at 25 °C for 5 minutes, 20 mL of each mixture is spotted on a piece of phosphocellulose disc which is immediately put into 1% H3PO4. After free [g-32P] ATP on the discs is removed, the peptide-incorporated 32P on the discs is counted in a scintillation counte
In vitro	在水迷宫测试和强迫游泳测试中,Daphnetin（2-8 mg/kg）可增强应激小鼠的表现. Daphnetin（140 mg/kg）可使子宫重量减少39.5%.Daphnetin可使P. yoelli感染小鼠的存活时间显著延长.
In vivo	Daphnetin（25-40 μM）对恶性疟原虫引起的次黄嘌呤掺入的抑制率可达50%。Daphnetin（50 μM）可使细胞周期蛋白D1的水平降低。地塞米松可影响皮质神经从而降低细胞活性,Daphnetin可剂量依赖地防止该作用。 Daphnetin能以特殊的方式剂量依赖性地抑制内源或重组TaPRK的活性。Daphnetin既对表皮细胞生长因子受体催化的外源底物酪氨酸磷酸化具有明显抑制作用,还对PKA和PKC的活性有抑制作用。Daphnetin处理24 h对MCF-7雌激素应答的人类癌细胞系生长有抑制作用（IC50：73 μM）。Daphnetin对ERK1/ERK2促有丝分裂的信号传导有抑制作用。
Storage	keep away from direct sunlight \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
Solubility Information	Ethanol : < 1 mg/mL (insoluble or slightly soluble) DMSO : 55 mg/mL (308.75 mM), Sonication is recommended. H2O : < 1 mg/mL (insoluble or slightly soluble)
Keywords	Autophagy \| PARP \| PKA \| A2780 cells \| mTOR \| Protein kinase A \| Inhibitor \| Caspase \| poly ADP ribose polymerase \| PKB \| inflammation \| schizontocidal \| Epidermal growth factor receptor \| Protein kinase C \| Reactive Oxygen Species \| HER1 \| Bcl-2 Family \| Parasite \| Protein kinase B \| Akt \| Mammalian target of Rapamycin \| AMPK \| Apoptosis \| protein kinase \| ErbB-1 \| Daphnetin \| inhibit \| AMP-activated protein kinase \| PKC \| EGFR
Inhibitors Related	Guanidine hydrochloride \| Naringin \| Valproic Acid \| Taurine \| Gefitinib \| Aceglutamide \| Doxycycline \| Hydroxychloroquine \| Stavudine \| Paeonol \| Sodium 4-phenylbutyrate \| Kojic acid
Related Compound Libraries	Neuroprotective Compound Library \| Inhibitor Library \| Kinase Inhibitor Library \| Anti-Aging Compound Library \| Membrane Protein-targeted Compound Library \| Tyrosine Kinase Inhibitor Library \| Antiparasitic Natural Product Library \| Drug Repurposing Compound Library \| Natural Product Library for HTS \| Anti-Cancer Drug Library

瑞香素|||Daphnetol|||7,8-Dihydroxycoumarin|TargetMol

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期	(12年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
主营行业	化学试剂,生物活性小分子