化合物 Wnt-C59|T2242|TargetMol

Wnt-C59
1243243-89-1

￥233 1mg 起订

￥329 2mg 起订

￥536 5mg 起订

上海更新日期：2024-09-23

TargetMol中国（陶术生物）

VIP12年

联系人：邵小姐

电话：021-021-33632979拨打

手机：15002134094 拨打

邮箱：marketing@targetmol.com

产品详情:

中文名称：: 化合物 Wnt-C59

英文名称：: Wnt-C59

CAS号：: 1243243-89-1

品牌：: TargetMol

产地：: 美国

保存条件：: store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.91%

产品类别：: 抑制剂

货号：: T2242

Product Introduction

Bioactivity

Name	Wnt-C59
Description	Wnt-C59 (C59) is a highly effective and specific Wnt signaling antagonis with PORCN enzymatic activity.
Cell Research	Wnt-C59 (C59) is dissolved in DMSO (10 mM) and stored, and then diluted with appropriate medium before use[1]. Approximately, 1×104 cells are seeded in 24-well plates, and Wnt-C59 (5 μM, 10 μM, and 20 μM) is added the next day. Each group is tested in triplicate and control groups with addition of DMSO are also established. Cell confluence is determined by microscopy at 24, 48, 72, and 96 hours after seeding of cells. The IC50 of Wnt-C59 is determined by MTT assay, using 96-well dishes. Next day, various concentrations of Wnt-C59 are added, and cellular viabilities are measured by a spectrophotometer at both 24 and 48 hours. For sphere formation, approximately one hundred cells are seeded onto the Low Cell Bind Surface 24-well Nunc dish. Each group is done in triplicate and each well had 2 mL medium. Media are changed twice a week, and only half of the media is changed each time. Approximately, 1×103 cells are seeded for each well in the sphere inhibition assay. At 1 to 5 days after plating, all tested cells formed small spheres. Five days later, Wnt-C59 (1 μM, 5 μM, and 20 μM) is added into experimental groups. Abilities for cell growth and sphere images are compared and recorded at the end of the first, second, and third weeks after addition of Wnt-C59, or DMSO in control groups. The sphere growths are observed and recorded daily under microscopy, and the area of spheres is analyzed using Metamorph and recorded as average area (μm2)[1].
Kinase Assay	Aurora A radioactive Flashplate enzyme assay: Aurora A radioactive Flashplate enzyme assay is conducted to determine the nature and degree of MLN8237-mediated inhibition in vitro. Recombinant Aurora A is expressed in Sf9 cells and purified with GST affinity chromatography. The peptide substrate for Aurora A is conjugated with biotin (Biotin-GLRRASLG). Aurora A kinase (5 nM) is assayed in 50 mM Hepes (pH 7.5), 10 mM MgCl2, 5 mM DTT, 0.05% Tween 20, 2 μM peptide substrate, 3.3 μCi/mL [γ-33P]ATP at 2 μM, and increasing concentrations of MLN8237 by using Image FlashPlates.
In vitro	在原位移植独立性MMTV-WNT1肿瘤的雌性裸鼠中,Wnt-C59（10 mg/kg）能够降低β-catenin靶向基因的表达,降低Wnt通路活性,抑制肿瘤细胞生长.14CREER/Rosa-SmoM2小鼠中,Wnt-C59（5 mg/kg）局部给药能够抑制细胞增殖.
In vivo	在转染PORCN的无PORCN HT1080细胞中,Wnt-C59（100 nM ）抑制PORCN活性。在转染WNT3A-V5的HeLa细胞中,Wnt-C59（10 -100 nM）能够抑制PORCN酰基转移酶活性。
Storage	store at low temperature,keep away from direct sunlight \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
Solubility Information	Ethanol : 7.6 mg/mL (20 mM) DMSO : 7.6 mg/mL (20 mM)
Keywords	Wnt \| WntC59 \| Inhibitor \| Porcupine \| Wnt-C59 \| Wnt-C-59 \| inhibit \| C 59 \| C-59 \| Wnt C59
Inhibitors Related	Urea \| Bisdemethoxycurcumin \| PRI-724 \| Nefopam hydrochloride \| XAV-939 \| EMT inhibitor-1 \| MSAB \| (E)-Ferulic acid \| FzM1 \| CHIR-99021 \| Wogonin \| Wnt pathway activator 1
Related Compound Libraries	Inhibitor Library \| Neuroprotective Compound Library \| Bioactive Compound Library \| Bioactive Compounds Library Max \| Anti-Colorectal Cancer Compound Library \| Osteogenesis Compound Library \| Stem Cell Differentiation Compound Library \| Orally Active Compound Library \| Neuronal Differentiation Compound Library \| Wnt/Hedgehog/Notch Compound Library

C59|||4-(2-甲基-4-吡啶基)-N-[4-(3-吡啶基)苯基]苯乙酰胺|TargetMol

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期	(12年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
主营行业	化学试剂,生物活性小分子