化合物 Y27632|T1870

Y-27632
146986-50-7

￥247 1mg 起订

￥352 2mg 起订

￥578 5mg 起订

上海更新日期：2024-12-02

TargetMol中国（陶术生物）

VIP12年

联系人：邵小姐

电话：021-021-33632979拨打

手机：15002134094 拨打

邮箱：marketing@targetmol.com

产品详情:

中文名称：: 化合物 Y27632

英文名称：: Y-27632

CAS号：: 146986-50-7

品牌：: TargetMol

产地：: 美国

保存条件：: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.87%

产品类别：: 抑制剂

货号：: T1870

Product Introduction

Bioactivity

名称	Y-27632
描述	ZY-27632 is an orally potent, ATP-competitive inhibitor of ROCK-I and ROCK-II. Y-27632 also inhibits isolation-induced apoptosis in mouse prostate stem or progenitor cells.
细胞实验	Y-27632 is dissolved in water and stored[1]. HeLa cells are plated at a density of 3×104 cells per 3.5-cm dish. The cells are cultured in DMEM containing 10% FBS in the presence of 10 mM Thymidine for 16 h. After the cells are washed with DMEM containing 10% FBS, they are cultured for an additional 8 h, and then 40 ng/mL of Nocodazole is added. After 11.5 h of the Nocodazole treatment, various concentrations of Y-27632 (0-300 μM), Y-30141, or vehicle is added and the cells are incubated for another 30 min[1].
激酶实验	Recombinant ROCK-I, ROCK-II, PKN, or citron kinase is expressed in HeLa cells as Myc-tagged proteins by transfection using Lipofectamine, and is precipitated from the cell lysates by the use of 9E10 monoclonal anti-Myc antibody coupled to G protein-Sepharose. Recovered immunocomplexes are incubated with various concentrations of [32P]ATP and 10 mg of histone type 2 as substrates in the absence or presence of various concentrations of either Y-27632 or Y-30141 at 30°C for 30 min in a total volume of 30 μL of the kinase buffer containing 50 mM HEPES-NaOH, pH 7.4, 10 mM MgCl2, 5 mM MnCl2, 0.02% Briji 35, and 2 mM dithiothreitol. PKCa is incubated with 5 μM [32P]ATP and 200 μg/mL histone type 2 as substrates in the absence or presence of various concentrations of either Y-27632 or Y-30141 at 30°C for 10 min in a kinase buffer containing 50 mM Tris-HCl, pH 7.5, 0.5 mM CaCl2, 5 mM magnesium acetate, 25 μg/mL phosphatidyl serine, 50 ng/mL 12-O-tetradecanoylphorbol-13-acetate and 0.001% leupeptin in a total volume of 30 μL. Incubation is terminated by the addition of 10 μL of 43 Laemmli sample buffer. After boiling for 5 min, the mixture is subjected to SDS-polyacrylamide gel electrophoresis on a 16% gel. The gel is stained with Coomassie Brilliant Blue, and then dried. The bands corresponding to histone type 2 are excised, and the radioactivity is measured[1].
体外活性	方法：人诱导多能干细胞 marmoset iPSC 用 Y-27632 (5-20 μM) 处理 7 天，使用 AKP 检测克隆形成情况。结果：Y-27632 显著提高 marmoset iPSC 的克隆效率。[1] 方法：成人脂肪组织衍生干细胞 ADSCs 用 Y-27632 (5 μmol/L) 处理 1 h，检测 ADSCs 的形态变化。结果：Y-27632 剂量依赖性诱导 ADSCs 的神经元分化，5 μmol/L Y-27632 处理 1 h 的 ADSCs的神经元样细胞百分比为（93.5±4.7）%。[2] 方法：食蟹猴胚胎干细胞 cyES 常规传代或用 Y-27632 (1-10 μM) 处理 24 h，使用 Flow Cytometry 方法进行活-死染色，使用试剂盒检测 BrdU。结果：Y-27632 促进 cyES 存活细胞增加。Y-27632 没有促进细胞增殖，但保护细胞在单细胞消化后免于细胞死亡。[3]
体内活性	方法：为研究 Y-27632 在运动神经元疾病的治疗潜力，将 Y-27632 (2 or 30 mg/kg in drinking water) 口服给 ALS 模型的 SOD1G93A 小鼠，持续 137 天。结果：Y-27632 2 mg/kg 治疗的效果不佳，Y-27632 30 mg/kg 治疗可改善雄性小鼠的运动功能，雌性小鼠仅表现出有限的改善。[4] 方法：为研究 Y-27632 对肝纤维化的影响，将 Y-27632 (30 mg/kg) 口服给药给 dimethylnitrosamine (DMN) 诱导肝纤维化的大鼠，每天一次，持续四周。结果：Y-27632 治疗显著减少了 DMN 诱导的肝纤维化的发生，并降低了肝脏中胶原和羟脯氨酸的含量以及 α-SMA 的表达。[5]
存储条件	keep away from direct sunlight \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	H2O : insoluble 10% DMSO+90% Saline : 2.47 mg/mL (9.99 mM), Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. DMSO : 45 mg/mL (181.94 mM)
关键字	Rho-associated protein kinase \| ROK \| Inhibitor \| inhibit \| Rho-kinase \| Apoptosis \| Y 27632 \| Rho-associated kinase \| Y27632 \| ROCK \| Y-27632
相关产品	L-Glutamic acid \| Metronidazole \| 5-Fluorouracil \| Dextran sulfate sodium salt (MW 4500-5500) \| Stavudine \| Tributyrin \| Myricetin \| Sorafenib \| L-Ascorbic acid \| Acetylcysteine \| Salicylic acid \| Sodium 4-phenylbutyrate
相关库	抑制剂库 \| 抗癌活性化合物库 \| 经典已知活性库 \| 已知活性化合物库 \| 细胞周期化合物库 \| 激酶抑制剂库 \| 神经再生化合物库 \| 细胞骨架化合物库 \| TGF-β/Smad靶点化合物库 \| 细胞重编程化合物库

Y-27632|TargetMol

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期	(12年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
主营行业	化学试剂,生物活性小分子