5-氟胞嘧啶|T0986

Flucytosine
2022-85-7

￥291 500mg 起订

上海更新日期：2024-12-02

TargetMol中国（陶术生物）

VIP12年

联系人：邵小姐

电话：021-021-33632979拨打

手机：15002134094 拨打

邮箱：marketing@targetmol.com

产品详情:

中文名称：: 5-氟胞嘧啶

英文名称：: Flucytosine

CAS号：: 2022-85-7

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.83%

产品类别：: 抑制剂

货号：: T0986

Product Introduction

Bioactivity

名称	Flucytosine
描述	Flucytosine (NSC 103805) is a pyrimidine compound and a fluorinated cytosine analog exhibiting antifungal activity.
细胞实验	J774.16 murine macrophage-like cells plated on 96-well tissue culture plates are incubated with 500 u/mL murine recombinant gamma-interferon overnight at 37 °C. The medium is then replaced with fresh medium containing gamma-interferon 500 μ/mL, LPS 3 mg/L, C. neoformans cells 1.6 × 104/well and Flucytosine 50 mg/L. Plates are incubated for 24 hours at 37 °C. Cell supernatants are collected and cells lysed by adding 0.1 mL sterile distilled water to each well, incubating at room temperature for 30 minutes, and then aspirating and ejecting the lysate with a pipette several times to complete cell disruption. Wells are rinsed with PBS (0.1 mL) and the cell supernatant, and lysate and rinse from each well are pooled, vortexed, diluted 1:50, vortexed again and spread on Sabouraud's dextrose agar for the cfu determination(Only for Reference)
激酶实验	Microdilution method: The culture media used are RPMI 1640 with glutamine, without bicarbonate and phenol red, buffered with morpholinopropanesulfonic acid (MOPS) (0.165 M, pH 7.0). Two-fold serial dilutions of Flucytosine (0.06-64 μg/mL) are prepared and dispensed in 50 uL aliquot, in flat-bottom 96-well assay plates which are kept frozen at -70 °C in sealed plastic bags until used. The inoculum is prepared spectrophotometrically and standardized to a concentration of 1.0-5.0 × 103 cfu per mL. A 50 μL volume of this suspension is used to inoculate each well containing 50 μL of the double concentration of Flucytosine to be tested. Once inoculated, each well therefore contains 100 μL of broth favoured over 200 μL to facilitate the agitation of the plates prior to spectrophotometric reading. After an incubation period of 24 and 48 hours at 35 °C, the plates are agitated for 3 minutes at 900 r.p.m. with a shaker and the optical density of the growth in each well is determined with the use of an automatic plate reader set at 495 nm. The inhibitory concentration of IC50 is computed mathematically.
体外活性	在感染新型隐球菌的A/JCr小鼠中,Flucytosine和单抗2H1联合处理效果比单独给药效果好,明显降低肺部菌落形成.
体内活性	Lucytosine（≥1.25 mg / L）抑制Sabouraud葡萄糖培养液中C. neoformans的生长。
存储条件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	DMSO : 3.85 mg/mL (29.79 mM), Sonication is recommended. H2O : 5 mg/mL (38.73 mM) Ethanol : < 1 mg/mL (insoluble or slightly soluble)
关键字	Antibiotic \| Fungal \| NSC103805 \| Inhibitor \| Flucytosine \| inhibit \| NSC-103805
相关产品	Cefaclor monohydrate \| Penicillin G sodium salt \| G-418 disulfate \| Doxycycline \| Neomycin sulfate \| Metronidazole \| Dexamethasone \| Chlorhexidine digluconate \| EDTA copper(II) disodium salt \| Ampicillin sodium \| Sulfamethoxazole sodium \| Kanamycin sulfate
相关库	经典已知活性库 \| 抗癌活性化合物库 \| 植物来源化合物库 \| 抗病毒库 \| FDA 上市药物库 \| 天然产物库 \| 高通量筛选天然产物库 \| 抗癌临床化合物库 \| 抗感染天然产物库 \| 抗癌药物库

Ro 2-9915|||NSC 103805|||Fluorocytosine|||5-氟胞嘧啶|||5-Fluorocytosine|TargetMol

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期	(12年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
主营行业	化学试剂,生物活性小分子