米哚妥林|T3211

Midostaurin
120685-11-2

￥627 1mg 起订

￥1490 5mg 起订

￥2380 10mg 起订

上海更新日期：2026-05-08

TargetMol中国（陶术生物）

VIP14年

联系人：邵小姐

电话：021-021-33632979拨打

手机：15002134094 拨打

邮箱：marketing@targetmol.com

产品详情:

中文名称：: 米哚妥林

英文名称：: Midostaurin

CAS号：: 120685-11-2

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

纯度规格：: 99.56%

产品类别：: 抑制剂

货号：: T001|T3211

Product Introduction

Bioactivity

名称	Midostaurin
描述	Midostaurin (PKC412) is a multi-targeted tyrosine kinase inhibitor with antitumor activity, exhibiting IC50 values ranging from 22 to 500 nM against PKCα/β/γ, Syk, Flk-1, Akt, PKA, c-Kit, c-Fgr, c-Src, FLT3, PDFRβ, and VEGFR1/2. Midostaurin is indicated for the treatment of acute myeloid leukemia (AML) and systemic mastocytosis (SM).
细胞实验	Each well is added with 5 mM WST-1 and 0.2 mM 1-methoxy PMS and the absorbance at 450 nm is measured by a Microplate Reader.(Only for Reference)
激酶实验	Cells are plated overnight and treated with 100 nM AZD3965 or vehicle for 24 hours. The cells are then washed, once in PBS and twice with lysis buffer (50 mM Mops, 100 mM KCl, 5 mM MgCl2, 1 mM EDTA, 0.1 mM DTT, 1 mM PMSF supplemented with 1× mini complete protease inhibitor cocktail tablets. The cells are harvested by scraping and centrifugation, and the pellet snap frozen without buffer in liquid nitrogen. Frozen aliquots of cells are thawed on ice and re-suspended in lysis buffer. Cells are lysed by 3 rounds of freezing in liquid nitrogen and thawing at 37°C for 2 minutes each. Lysates are subsequently centrifuged (13000 g, 10min, 4°C) until clear and then stored on ice. Enzyme activity in the cell lysates is determined using a micro-plate reader to measure either production or depletion of NADH/NADPH, through its absorbance at 340/10 nm, occurring as a result of direct or coupled enzyme reactions. The 96 well plates used for the assays are pre-heated to 37°C for 10 minutes prior to starting reactions, initiated by the addition of 5 μL cell lysate to 95 μL of reaction buffer (50 mM Mops pH 7.4, 100 mM KCl, 5 mM free magnesium). The standard reaction buffer is supplemented to assay the kinetics of the different enzymes. Absorbance values for controls are subtracted from absorbance of corresponding reactions. Graphpad prism 6 is used to plot V0 values against substrate concentration and determine Vmax and Km values. The Vmax is then normalised to the protein concentration in the cell lysate[1].
体外活性	方法：肥大细胞白血病细胞系 HMC-1.1，加入 Midostaurin（1 μM），处理 24-48 小时，TUNEL 染色检测细胞凋亡情况。结果：Midostaurin 能显著诱导细胞凋亡。[1] 方法： K562-FLT3 细胞（在 K562 细胞中过表达 FLT3）加入浓度梯度 Midostaurin （ 0.001 - 10 μM）处理，处理 72 小时。结果：Midostaurin 对 K562-FLT3 细胞有显著的抑制作用。[2]
体内活性	方法：将 K562-FLT3 细胞皮下接种于免疫缺陷小鼠。待肿瘤可触及后，随机分组开始给药。Midostaurin 50 mg/kg，每日一次，口服灌胃。 Imatinib 50 mg/kg，每日两次，口服灌胃。联合治疗组同时给予上述剂量的 Midostaurin 和 Imatinib。结果：联合治疗组小鼠的肿瘤生长被显著抑制。[2]
存储条件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.
溶解度	10% DMSO+40% PEG300+5% Tween 80+45% Saline : 5 mg/mL (8.76 mM), Sonication is recommended. DMSO : 247.5 mg/mL (433.72 mM), Sonication is recommended.
关键字	VEGFR \| Vascular endothelial growth factor receptor \| SCFR \| Protein kinase C \| PPK \| PKCγ \| PKCβ2 \| PKCβ1 \| PKCα \| PKC-412 \| PKC 412 \| PKC \| NOS \| NO Synthase \| Nitric oxide synthases \| Midostaurin \| Inhibitor \| inhibit \| c-Kit \| CGP-41251 \| CGP41251 \| CGP-41231 \| CGP 41231 \| CD117 \| Apoptosis
相关产品	Formamide \| Urea \| Dimethyl phthalate \| Aceglutamide \| Alginic acid \| Cysteamine hydrochloride \| Metronidazole \| Sildenafil citrate \| Citric Acid Triammonium \| Stavudine \| Gluconate Calcium \| Tamoxifen
相关库	经典已知活性库 \| 抗癌活性化合物库 \| 激酶抑制剂库 \| 临床失败化合物库 \| 抗病毒库 \| TGF-β/Smad靶点化合物库 \| 免疫/炎症分子化合物库 \| 膜蛋白靶向化合物库 \| 药物功能重定位化合物库 \| 酪氨酸激酶分子库 \| 抗癌临床化合物库 \| 抗癌药物库

米哚妥林;苯甲酰基十字孢碱;PKC412;N-Benzoylstaurosporine;CGP41231;CGP 41251;TargetMol

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期	(14年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
主营行业	化学试剂,生物活性小分子