罗米地辛|T6006

Romidepsin
128517-07-7

￥832 1mg 起订

￥1330 2mg 起订

￥2430 5mg 起订

上海更新日期：2024-12-02

TargetMol中国（陶术生物）

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联系人：邵小姐

电话：021-021-33632979拨打

手机：15002134094 拨打

邮箱：marketing@targetmol.com

产品详情:

中文名称：: 罗米地辛

英文名称：: Romidepsin

CAS号：: 128517-07-7

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.67%

产品类别：: 抑制剂

货号：: T6006

Product Introduction

Bioactivity

名称	Romidepsin
描述	Romidepsin (FR 901228) is an HDAC inhibitor that inhibits HDAC1/2/4/6 (IC50=36/47/510/1400 nM). Romidepsin has antitumor activity and can be used for the treatment of peripheral T-cell lymphoma and cutaneous T-cell lymphoma.
细胞实验	Cells are exposed to various concentrations of Romidepsin for 72 hours in 96-well plates. 20 μL of 5 mg/mL MTT solution in PBS is added to each well for 4 hours. After removal of the medium, 170 μL of DMSO is added to each well to dissolve the formazan crystals. The absorbance at 540 nm is determined. In addition, cells are incubated with trypan blue, and the numbers of blue (dead) cells and transparent (live) cells are counted in a hemocytometer. For cell cycle analysis, cells are incubated for 30 minutes in propidium iodide staining solution containing 0.05 mg/mL propidium iodide, 1 mM EDTA, 0.1% Triton X-100, and 1 mg/mL RNase A in PBS. The suspension is then passed through a nylon mesh filter and analyzed on a Becton Dickinson FACScan. (Only for Reference)
激酶实验	HDAC-inhibitory activity: For the enzyme assay, 10 μL of [3H]acetyl-labeled histones (25,000 cpm/10 μg) are added to 90 μL of the HDAC enzyme fraction extracted from 293T cells overexpressing HDAC1 or HDAC2 in the presence of increasing concentrations of Romidepsin, and the mixture is incubated at 37 °C for 15 minutes. The enzyme reaction is linear for at least 1 hour. The reaction is stopped by the addition of 10 μL of concentrated HCl. The released [3H]acetic acid is extracted with 1 mL of ethylacetate, and 0.9 mL of the solvent layer is taken into 5 mL of aqueous counting scintillant II solution for determination of radioactivity. The IC50 values are determined from at least three independent dose-response curves.
体外活性	方法：两种恶性 T 细胞系 PEER 和 SUPT1 用 Romidepsin (2.5-40 nM) 处理 48 h，使用 MTT Assay 检测细胞活力。结果：Romidepsin 抑制 PEER 和 SUPT1细胞活力，IC50 值为 10.8 nM 和 7.9 nM。[1] 方法：CD20+ rituximab 敏感细胞 Raji 细胞和耐药细胞 Raji-2R、Raji-4RH 用 Romidepsin (10 ng/mL) 处理 2 天，使用 Flow Cytometry 检测胞内 caspase 3 激活水平。结果：Raji 细胞中，Romidepsin 增加了 active caspase 3。在 Raji-2R 和 Raji-4RH 中未检测到 cleaved active caspase 3。[2]
体内活性	方法：为检测体内抗肿瘤活性，将 Romidepsin (4.4 mg/kg) 腹腔注射给携带人 Burkitt 淋巴瘤 Raji 和 Raji-2R 的 NSG 小鼠，每周一次，持续三周。结果：Romidepsin 显著抑制异种移植小鼠中 Raji 和 Raji-2R 细胞的生长。[2] 方法：为检测体内抗肿瘤活性，将 Romidepsin (0.03 mg/mouse，0.5% methylcellulose) 腹腔注射给 DEN 诱导肝癌的 C56BL/6 小鼠，每周两次，连续三周。结果：Romidepsin 抑制肿瘤进展，这种效应与肿瘤细胞增殖减少和凋亡增加有关。[3]
存储条件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	H2O : Insoluble DMSO : 100 mg/mL (184.9 mM)
关键字	FR-901228 \| HDAC \| NSC-630176 \| Histone deacetylases \| FK228 \| Apoptosis \| NSC630176 \| Inhibitor \| inhibit \| FK-228 \| FR901228 \| Romidepsin
相关产品	Valproic Acid \| L-Glutamic acid \| Metronidazole \| 5-Fluorouracil \| Curcumin \| Dextran sulfate sodium salt (MW 4500-5500) \| Stavudine \| Tributyrin \| L-Ascorbic acid \| Acetylcysteine \| Salicylic acid \| Sodium 4-phenylbutyrate

罗米地辛|||NSC 630176|||FR 901228|||FK 228|||Depsipeptide|TargetMol

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期	(12年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
主营行业	化学试剂,生物活性小分子