化合物 Torin 1|T6045

Torin 1
1222998-36-8

￥273 1mg 起订

￥389 2mg 起订

￥637 5mg 起订

上海更新日期：2024-12-02

TargetMol中国（陶术生物）

VIP12年

联系人：邵小姐

电话：021-021-33632979拨打

手机：15002134094 拨打

邮箱：marketing@targetmol.com

产品详情:

中文名称：: 化合物 Torin 1

英文名称：: Torin 1

CAS号：: 1222998-36-8

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.38%

产品类别：: 抑制剂

货号：: T6045

Product Introduction

Bioactivity

名称	Torin 1
描述	Torin 1 is an mTOR inhibitor that inhibits mTORC1 and mTORC2 (IC50=2/10 nM) selectively and ATP-competitively. Torin 1 induces cellular autophagy and possesses anti-tumor, anti-inflammatory, and anti-aging activities.
细胞实验	Cell viability is assessed with the CellTiter-Glo Luminescent Cell Viability Assay. On Day 0, 96-well plates are seeded with 500 cells per well and grown overnight. On Day 1, cells are treated with the appropriate compounds and subsequently analyzed on Days 3-5. For analysis, plates are incubated for 60 min at room temperature; 50 μL of CellTiter-Glo reagent is added to each well, and plates are mixed on an orbital shaker for 12 min. Luminescence is quantified on a standard plate luminometer. (Only for Reference)
激酶实验	mTORC1 and mTORC2 in Vitro Kinase Assays: To produce soluble mTORC1, HEK-293T cell lines that stably express N-terminally FLAG-tagged Raptor are generated using vesicular stomatitis virus G-pseudotyped MSCV retrovirus. For mTORC2, similar HeLa cells that stably express N-terminally FLAG-tagged Protor-1 are generated. Both complexes are purified by lysing cells in 50 mm HEPES, pH 7.4, 10 mm sodium pyrophosphate, 10 mm sodium β-glycerophosphate, 100 mm NaCl, 2 mm EDTA, 0.3% CHAPS. Cells are lysed at 4 °C for 30 min, and the insoluble fraction is removed by microcentrifugation at 13,000 rpm for 10 min. Supernatants are incubated with FLAG-M2 monoclonal antibody-agarose for 1 h and then washed three times with lysis buffer and once with lysis buffer containing a final concentration of 0.5 M NaCl. Purified mTORC1 is eluted with 100 μg/mL 3×FLAG peptide in 50 mm HEPES, pH 7.4, 100 mm NaCl. Eluate can be aliquoted and stored at -80 °C. Substrates S6K1 and Akt1 are purified. Kinase assays are performed for 20 min at 30 °C in a final volume of 20 μL consisting of the kinase buffer (25 mm HEPES, pH 7.4, 50 mm KCl, 10 mm MgCl2, 500 μm ATP) and 150 ng of inactive S6K1 or Akt1 as substrates. Reactions are stopped by the addition of 80 μL of sample buffer and boiled for 5 min. Samples are subsequently analyzed by SDS-PAGE and immunoblotting.
体外活性	方法：野生型 MEFs 细胞用 Torin 1 (250 nm) 处理 4 天，使用 CellTiterGlo viability assay 检测细胞增殖。结果：250 nm Torin1 完全抑制细胞增殖。[1] 方法：人类内分泌细胞系 BON 用 Torin 1 (62.5-150 µM) 处理 3-24 h，使用 Western Blot 检测靶点蛋白表达水平。结果：Torin1 以剂量依赖的方式增加 NT 分泌；在 Torin1 处理后 3-24 h，NT 分泌增加。Torin1 处理降低了 Akt (S473) 磷酸化，p70S6K (T389) 以及 4E-BP1 (T37/46) 的磷酸化也受到抑制。[2]
体内活性	方法：为检测体内抗肿瘤活性，将 Torin 1 (20 mg/kg，20% N-mentyl-2-pyrrolidone, 40% PEG400 and 40% water) 腹腔注射给携带人胶质母细胞瘤 U87MG 的 NCR nude 小鼠，每天一次，持续十天。结果：连续给药十天导致大于 99% 的肿瘤生长抑制。停止药物治疗后，肿瘤继续生长，这表明 Torin 1 治疗主要是细胞抑制性的，并且大量肿瘤细胞在治疗过程中仍然存活。[3]
存储条件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	10% DMSO+90% Saline : 0.15 mg/mL (0.25 mM), Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. DMSO : 1 mg/mL, Sonication is recommended.
关键字	Mammalian target of Rapamycin \| mTOR \| Autophagy \| Torin1 \| Inhibitor \| Torin-1 \| Torin 1 \| inhibit
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Torin 1|TargetMol

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期	(12年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
主营行业	化学试剂,生物活性小分子