雷西莫特|T6964

Resiquimod
144875-48-9

￥143 1mg 起订

￥297 5mg 起订

￥452 10mg 起订

上海更新日期：2025-03-12

TargetMol中国（陶术生物）

VIP12年

联系人：邵小姐

电话：021-021-33632979拨打

手机：15002134094 拨打

邮箱：marketing@targetmol.com

产品详情:

中文名称：: 雷西莫特

英文名称：: Resiquimod

CAS号：: 144875-48-9

品牌：: TargetMol

产地：: 美国

保存条件：: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.95%

产品类别：: 抑制剂

货号：: T6964

Product Introduction

Bioactivity

名称	Resiquimod
描述	Resiquimod (R848) is a Toll-like receptor 7/8 (TLR7/TLR8) agonist that induces cytokine upregulation. Resiquimod is an immunomodulator with antitumor and antiviral activity.
细胞实验	Resiquimod is dissolved in DMSO. For inhibition of lysosomal acidification, cells are incubated with 10?μM CQ for 1?h before Resiquimod (R848) treatment. After treatment, 20?μL of 5?mg/mL MTT is added to the plate. The plate is incubated at 22°C for 4?h, and 200?μL dimethyl sulfoxide is added to the plate to dissolve the reduced formazan. The plate is then read at 490?nm with a microplate reader. To determine the effect of Myd88 inhibition on R848-induced cell proliferation, the Myd88 inhibitor Pepinh-MYD and the control peptide Pepinh-Control are added to PBL at the concentration of 50?μM, and the plate is incubated at 22°C for 6?h. After incubation, the cells are treated with R848 and subjected to MTT assay as above. To determine the effect of NF-κB inactivation on R848-induced cell proliferation, BAY-11-7082, an irreversible inhibitor of IκB-α phosphorylation, is added to the cells at the concentration of 1?μM, and the plate is incubated at 22°C for 1?h. After incubation, the cells are treated with R848 and subjected to MTT assay as earlier. All experiments are performed three times.
激酶实验	For luciferase assay, FG-9307 cells are transfected with the firefly NF-κB-specific luciferase reporter vector pNFκB-Met-Luc2. Transfection efficiency is monitored by co-transfection with the pSEAP2 control vector, which constitutively expresses the human secreted enhanced alkaline phosphatase (SEAP). Then the cells are treated with Resiquimod (R848, 1?μg/mL), CQ (10?μM), CQ plus R848 or PBS and incubated at 22°C for 24?h. The culture medium of the transfectants is then analyzed for luciferase activity and SEAP activity using Luciferase Assay Kit and the Great EscAPe? SEAP Chemiluminescence Detection Kit, respectively. The assay is performed three times.
动物实验	Animal Models: Wild-type mice,TLR7-deficient mice,and MyD88-deficient mice. Formulation: saline. Dosages: 50 nmol. Administration: i.p.
体外活性	方法: 金绒球头肾淋巴细胞 HKL 用 Resiquimod (0.175-32 µg/mL) 处理 12 h，使用 CCK8 assay 检测细胞活力。结果: CCK8 测定结果显示，0.25-32 µg/mL Resiquimod 显著促进 HKL 增殖。[1] 方法: 外周血单核细胞 PBM 用 Resiquimod (0.01-100 µM) 处理 1-14 h，使用 Western Blot 方法检测靶点蛋白表达水平。结果: 1 µM 的剂量足以改变 FcγR 的表达，更高的剂量不会导致更大的变化。FcγRIIa 的增加发生在晚期，而 γ 链的小幅增加出现在 3 h，但在 14 h 时更高。然而，FcγRIIb 蛋白在1 h 内减少，而 FcγRIIb 的转录物保持到 4 h。[2]
体内活性	方法: 为检测体内抗肿瘤活性，将 Resiquimod (2 mg/kg) 和 4D5 抗 HER2 抗体 (20 mg/kg) 腹腔注射给携带 CT26-HER2/neu 肿瘤的 Balb/cJ 小鼠，每周三次，持续 13 天。结果: 13 天后，接受 Resiquimod 加抗体的小鼠肿瘤的生长速率显著降低。统计测试显示 4D5 和 Resiquimod 在降低肿瘤生长速率方面具有协同作用。[2]
存储条件	store at low temperature \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	H2O : < 1 mg/mL (insoluble or slightly soluble) 10% DMSO+90% Saline : 0.1 mg/mL (0.32 mM), Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. DMSO : 55 mg/mL (174.95 mM) Ethanol : 20 mg/mL (63.6 mM)
关键字	Hepatitis C virus \| R 848 \| S 28463 \| R-848 \| S-28463 \| Toll-like Receptor (TLR) \| Resiquimod \| Inhibitor \| inhibit \| HCV
相关产品	Chloranil \| Chloroquine phosphate \| Honokiol \| Hydroxychloroquine \| Artemisinin \| EIDD-1931 \| CU-115 \| Methyl 2-amino-5-bromobenzoate \| Chloroquine \| Deferiprone \| Ribavirin \| Sofosbuvir
相关库	经典已知活性库 \| 抗癌活性化合物库 \| 抗病毒库 \| 膜蛋白靶向化合物库 \| 药物功能重定位化合物库 \| 抗癌药物库

雷西莫特|||S28463|||R848|TargetMol

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期	(12年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
主营行业	化学试剂,生物活性小分子