米托蒽醌|T6588

Mitoxantrone
65271-80-9

￥297 25mg 起订

￥418 50mg 起订

￥617 100mg 起订

上海更新日期：2024-09-14

TargetMol中国（陶术生物）

VIP12年

联系人：邵小姐

电话：021-021-33632979拨打

手机：15002134094 拨打

邮箱：marketing@targetmol.com

产品详情:

中文名称：: 米托蒽醌

英文名称：: Mitoxantrone

CAS号：: 65271-80-9

品牌：: TargetMol

产地：: 美国

保存条件：: keep away from direct sunlight,store at low temperature,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 98.74%

产品类别：: 抑制剂

货号：: T6588

Product Introduction

Bioactivity

名称	Mitoxantrone
描述	Mitoxantrone (mitozantrone) is an inhibitor of topoisomerase II (Topo II), an inhibitor of protein kinase C (PKC) (IC50=8.5 μM). Mitoxantrone has antitumor activity for the treatment of acute myeloid leukemia, hepatocellular carcinoma, and breast cancer.
细胞实验	The human breast carcinoma cell lines MDA-MB-231 and MCF-7 are seeded in standard 96-well plates. One day after seeding, the culture medium is changed and replaced by medium containing different concentration of Mitoxantrone (10-5 to 5 μM) with or without DHA (30 μM) during 7 days. Viability of cells are measured as a whole by the tetrazolium salt assay[3].
激酶实验	activity-based protein profiling (ABPP): Mouse brains are Dounce-homogenized in PBS, pH7.5, followed by a low-speed spin (1,400×, 5 min) to remove debris. The supernatant is then subjected to centrifugation (64,000×, 45 min) to provide the cytosolic fraction in the supernatant and the membrane fraction as a pellet. The pellet is washed and resuspended in PBS buffer by sonication. Total protein concentration in each fraction is determined using a protein assay kit. Samples are stored at -80 °C until use. Mouse brain membrane proteomes, are diluted to 1 mg/mL in PBS and pre-incubated with varying concentrations of inhibitors (1 nM to 10 mM) for 30 min at 37 °C before the addition of FP-rhodamine at a final concentration of 2 mM in a 50 mL total reaction volume. After 30 min at 25 °C, the reactions are quenched with 4×SDS-PAGE loading buffer, boiled for 5 min at 90 °C, subjected to SDS-PAGE and visualized in-gel using a flatbed fluorescence s
体外活性	方法：人乳腺癌细胞 MCF7 用 Mitoxantrone (0.07-9.36 µM) 处理 48 h，使用 SRB assay 测定细胞活力。结果：MCF7 细胞在 Mitoxantrone 1.17 µM 剂量下达到对应于 IC50 的抑制作用。[1] 方法：骨肉瘤细胞 U2OS 用 Mitoxantrone (0.2-1 µM) 处理 48 h，使用 Western Blot 检测靶点蛋白表达水平。结果：Mitoxantrone 处理增加了 Caspase-3 和 PARP1 的裂解。Mitoxantrone 处理还增加了骨肉瘤细胞中 Bax 和 Bim EL 的表达。然而，Mitoxantrone 处理降低了 Bcl-2 的表达。[2]
体内活性	方法：为测试对，将 Mitoxantrone (0.4-3.2 mg/kg) 腹腔注射给注射白血病细胞 L1210 的 CDF1 小鼠，每天一次。结果：以最佳剂量 (1.6 mg/kg/天) 给予 Mitoxantrone 产生了具有统计学意义的 60 天存活率。[3]
存储条件	keep away from direct sunlight,store at low temperature,keep away from moisture \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	Ethanol : < 1 mg/mL (insoluble or slightly soluble) DMSO : 88 mg/mL (197.98 mM) H2O : < 1 mg/mL (insoluble or slightly soluble)
关键字	leukaemia \| NSC301739 \| PKC \| MCF-7 \| NSC 301739 \| lymphocytes \| Topoisomerase \| HL60 cells \| NSC-301739 \| B-CLL cells \| Protein kinase C \| inhibit \| Orthopoxvirus \| antitumor \| Endogenous Metabolite \| Mitoxantrone \| Apoptosis \| breast cancer \| Inhibitor
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相关库	抑制剂库 \| 经典已知活性库 \| 抗癌活性化合物库 \| 已知活性化合物库 \| 激酶抑制剂库 \| FDA 上市药物库 \| TGF-β/Smad靶点化合物库 \| 药物功能重定位化合物库 \| 疼痛相关化合物库 \| 抗癌药物库

米托蒽醌|||mitozantrone|TargetMol

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期	(12年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
主营行业	化学试剂,生物活性小分子