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  • 鹅去氧胆酸|T0847

鹅去氧胆酸|T0847

Chenodeoxycholic acid
474-25-9
￥190 20mg 起订
上海 更新日期：2024-12-02

TargetMol中国（陶术生物）

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产品详情:

中文名称：
鹅去氧胆酸
英文名称：
Chenodeoxycholic acid
CAS号：
474-25-9
品牌：
TargetMol
产地：
美国
保存条件：
Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
纯度规格：
99.97%
产品类别：
抑制剂
货号：
T0847

Product Introduction

Bioactivity

名称Chenodeoxycholic acid
描述Chenodeoxycholic acid (CDCA) is a bile acid, usually conjugated with either glycine or taurine. It acts as a detergent to solubilize fats for intestinal absorption and is reabsorbed by the small intestine. It is used as cholagogue, a choleretic laxative, and to prevent or dissolve gallstones.
细胞实验The cell viability is analyzed by incubating transfected HEK-293 cells and CHO cells for 1 h with the corresponding concentration of bile acid and staining with trypan blue. The toxicity of bile acids is analyzed using the tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) according to the cell proliferation kit I. No significant differences between control and bile acid-treated cells are obtained in both tests.
激酶实验Briefly, transfected HEK-293 cells, incubated in charcoal-treated Dulbecco's modified Eagle's medium for 24 h, are washed once with Hanks' solution and resuspended in a buffer containing 100 mM NaCl, 1 mM MgCl2, 1 mM EDTA, 1 mM EGTA, 250 mMsucrose, 20 mM Tris-HCl, pH 7.4. Cells are lysed by freezing in liquid nitrogen. Dehydrogenase activity is measured in a final volume of 20 μL containing the appropriate concentration of bile acid, 30 nCi of [3H]cortisol, and unlabeled cortisol to a final concentrations of 50 nM. The reaction is started by mixing cell lysate with the reaction mixture. Alternatively, endoplasmic reticulum microsomes are prepared from transfected HEK-293 cells and incubated with reaction mixture containing various concentrations of cortisol and CDCA. Incubation proceeded for 20 min, and the conversion of cortisol to cortisone is determined by thin layer chromatography (TLC). Because of the inaccuracy of the TLC method at low conversion rates and the end-product inhibition of 11βHSD2 at conversion rates higher than 60-70%, only conversion rates between 10 and 60% are considered for calculation. The inhibitory constant IC50 is evaluated using the curve-fitting program. Results are expressed as means±S.E. and consist of at least four independent measurements.
体外活性Chenodeoxycholic acid （CDCA）和脱氧胆酸（DCA）均可抑制11 beta HSD2，其IC(50)值分别为22 mM和38 mM。Chenodeoxycholic acid 诱导皮质醇依赖性核内转位，增加矿物皮质激素受体（MR）的转录活性。[1] Chenodeoxycholic acid 通过激活膜G蛋白偶联受体（TGR5）依赖性通路，显著促进Ishikawa细胞生长，增加Cyclin D1蛋白和mRNA表达。[2] 在培养的人类肝母细胞瘤细胞系Hep G2中，Chenodeoxycholic acid 可使LDL受体mRNA水平提高近4倍，HMG-CoA还原酶和HMG-CoA合酶mRNA水平提高2倍。[3] Chenodeoxycholic acid 诱导的Isc被Bumetanide、BaCl2和囊性纤维化跨膜导电调节器（CFTR）抑制剂CFTRinh-172抑制（≥67%）。Chenodeoxycholic acid 激活的Isc被腺苷酸环化酶抑制剂MDL12330A降低43%，Chenodeoxycholic acid 增加细胞内cAMP浓度。[4] Chenodeoxycholic acid 通过增加其磷酸化、核内积聚来激活C/EBPβ。Chenodeoxycholic acid 增强了含有C/EBP响应元素（pGL-1651）的-1.65-kb GSTA2启动子结构的荧光素酶基因转录。Chenodeoxycholic acid 激活AMP激活的蛋白激酶（AMPK），促进细胞外信号调节激酶1/2（ERK1/2）的激活。[5]
存储条件Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
溶解度Ethanol : 79 mg/mL (201.23 mM)
DMSO : 40 mg/mL (101.89 mM), Sonication is recommended.
H2O : < 1 mg/mL (insoluble or slightly soluble)
关键字NR1H4 | Endogenous Metabolite | Inhibitor | Chenodeoxycholic acid | inhibit | Autophagy | FXR
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相关库经典已知活性库 | 抗癌上市药物库 | EMA 上市药物库 | 疼痛相关化合物库 | 高通量筛选天然产物库 | 抗癌临床化合物库 | 抗癌药物库
鹅去氧胆酸|||Chenodiol|||CDCA|TargetMol

公司简介

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。 从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。 经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期 (12年)
注册资本 566.2651万人民币
员工人数 100-500人
年营业额 ￥ 1亿以上
经营模式 贸易,试剂,定制,服务
主营行业 化学试剂,生物活性小分子

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