重组人CD73 / NT5E蛋白-ACROBiosystems百普赛斯

Human CD73 / NT5E Protein, His Tag (HPLC-verified) (active enzyme)

分子别名（Synonym）

CD73,NT5E,5'-Nucleotidase,5'-NT,NT5,NTE

表达区间及表达系统（Source）

Human CD73 Protein, His Tag (CD3-H52H7) is expressed from human 293 cells (HEK293). It contains AA Trp 27 - Ser 549 (Accession # P21589-1).

Predicted N-terminus: Trp 27

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 59.9 kDa. The protein migrates as 65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-HPLC.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 120 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 24 months in lyophilized state;

-70°C for 12 months under sterile conditions after reconstitution.

背景（Background）

5'-核苷酸酶（5'-NT），也称为胞外-5'-核苷酸酶或CD73（分化簇73），是由NT5E基因编码的酶。CD73通常用于将AMP转化为腺苷。外切5-三核苷酸酶（5-核糖核苷酸磷酸水解酶）在中性pH下催化嘌呤5-三核苷酸转化为核苷，优选的底物是AMP。其他形式的5-三聚核苷酸酶存在于细胞质和溶酶体中，可以通过其底物亲和力、对二价镁离子的需求、ATP的激活和无机磷酸盐的抑制与胞外NPT5区分开来。罕见的等位基因变异与成人发病的关节和动脉钙化综合征（CALJA）有关，该综合征影响髂、股和胫动脉，减少腿部和手脚关节的循环，导致疼痛。