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  • 盐酸强力霉素|T1687L

盐酸强力霉素|T1687L

Doxycycline (hyclate)
24390-14-5
￥5450.00 100瓶 起订
￥326.00 100瓶 起订
￥620.00 5瓶 起订
￥395.00 1瓶 起订
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上海 更新日期：2024-09-14

TargetMol中国（陶术生物）

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联系人：邵小姐
电话：021-021-33632979拨打
手机：15002134094 拨打
邮箱：marketing@targetmol.com

产品详情:

中文名称：
盐酸强力霉素
英文名称：
Doxycycline (hyclate)
CAS号：
24390-14-5
品牌：
TargetMol
产地：
美国
保存条件：
Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
纯度规格：
99.94%
产品类别：
抑制剂
货号：
T1687L

Product Introduction

Bioactivity

名称Doxycycline (hyclate)
描述Doxycycline hyclate (WC2031) belongs to the tetracycline class of antibiotics and is a broad-spectrum metalloproteinase (MMP) inhibitor with oral activity. Doxycycline hyclate has antibacterial activity and antitumor activity.
细胞实验Doxycycline is diluted in culture medium at a concentration of 10 μg/mL. All in vitro treatments are performed in SMC cultures at 90% confluence, when ECM synthesis in SMC starts to be evident. Doxycycline (20 nM) is diluted in culture medium at a concentration of 10 μg/mL (20 nM), at which no toxicity or variation in primary cultured SMC proliferation has been reported, as well as in other cell lines and the incubation time is 48 h. SMC-C and SMC-Ch are seeded at equal cell density in 6-well plates and, when confluence reaches 90%, 1 mL culture medium is added to each well containing 3.7×104?Bq L-[5-3H]-proline (9.62×1011 Bq/mmol). After 48?h incubation, cells are lysed with 0.5 mL 0.5 mol/L NaOH for 1 h. The resulting solution is neutralized with an equal amount of 0.5 mol/L HCl, and 50 μL are used to measure total proteins with the Bradford method. One volume of 10% TCA is added to the remaining 250 μL and centrifuged at 13,000 g for 15?min at 4°C. The resulting precipitate is dissolved in 100 μL 0.2 mol/L NaOH, and then neutralized with 1 mol/L HCl. The solution is incubated with collagenase buffer (Tris-HCl, pH 7.6 20 mM, and CaCl2 250 mM final concentration) and 10 units of collagenase at 37°C overnight. Then, 150 μL 10% TCA are added and centrifuged at 13000 g for 15?min at 4°C. The resulting supernatant is added to 4 mL of scintillation fluid and the radioactivity is measured in a liquid scintillation counter LS 600 TA.
激酶实验Gelatin (0.1% (w/v) is added to standard LaemmLi acrylamide polymerization mixture. Tissue extract is mixed 1:2 with sample buffer [250 mM Tris-Cl pH 6.8, 10% (w/v) SDS, 20% (v/v) glycerol, 0.005% (w/v) bromphenol blue]. Serum is diluted 1:10 with electrophoresis buffer (2.5 mM Tris, 20 mM glycine, 0.005% SDS) and mixed 1:2 with sample buffer. Twenty μLs are loaded after 10-min incubation at room temperature without boiling. After electrophoresis at 90 V, the gels are soaked in 2.5% (w/v) Triton X-100, incubated 2 to 3 days at 37°C in gelatin digestion buffer [50 mM Tris-Cl, pH 8.0, 8 mM CaCl2, 10 mM ZnSO2, 0.02% (w/v) NaN3], stained in 0.05% Coomassie blue R-250 in acetic acid/methanol/water (1:4.5:4.5 by volume), destained in 10% acetic acid and 5% methanol, and scanned for lysis band intensity. The lysis band intensity is proportional to gelatinase activity and is quantified densitometrically by using One-Dimensional Scan software. The result, a number between 0.07 and 3.75, is normalized to the protein content by dividing the densitometry result with the relative optical density from the BCA protein assay kit result. The result is used for the analysis as the arbitrary unit. For the total MMP activity results of lysis bands of pro-MMP-9, active MMP-9, pro-MMP-2, and active MMP-2 are added. A protein size marker is used to determine the correct size.
体外活性方法：22 种肿瘤细胞用 Doxycycline hyclate 处理 48 h，使用 MTT 方法检测细胞活力。 结果：肺癌细胞对 Doxycycline 比其他大多数细胞系更敏感。NCI-H446 和 A549 细胞对 Doxycycline 敏感，IC50分别为 1.70 μM 和 1.06 μM。[1] 方法：人胰腺癌细胞 PANC-1 用 Doxycycline hyclate (10-40 μg/mL) 处理 8-24 h，使用 Flow Cytometry 方法检测细胞周期情况。 结果：Doxycycline 使细胞停滞在细胞周期的 G1-S 期。[2]
体内活性方法：为检测体内抗肿瘤活性，将 Doxycycline hyclate (50 mg/kg) 腹腔注射给携带人乳腺癌肿瘤 4T1 的 BALB/c 小鼠，每天一次，持续十五天。 结果：Doxycycline 延迟肿瘤生长并降低肿瘤相关巨噬细胞和血管的数量。[3] 方法：为检测体内抗菌活性，将 Doxycycline hyclate (40 mg/kg) 腹腔注射给暴露于 B. pseudomallei 1026b 的 BALB/c 小鼠，每天两次，持续两周。 结果：用 Doxycycline 治疗的小鼠 100% 存活，并且从大多数存活小鼠的肺或脾脏中没有扩增出 B. pseudomallei 的 DNA。[4]
存储条件Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
溶解度H2O : 13.89 mg/mL, Sonication is recommended.
DMSO : 55 mg/mL (107.23 mM), Sonication is recommended.
关键字U343 | anti-proliferative activity | SVG | MCF 12A | WC-2031 | antibacterial activity | Matrix metalloproteinases | 293T | LNT-229 | hyclate | inhibit | Doxycycline (hyclate) | WC 2031 | Antibiotic | MMP | Doxycycline Hydrochloride | Inhibitor | Bacterial | G55 | Doxycycline
相关产品Doxycycline | Neomycin sulfate | Dimethyl sulfoxide | Sulfamethoxazole sodium
相关库抑制剂库 | 微生物天然产物库 | 血管生成库 | 经典已知活性库 | 已知活性化合物库 | 天然产物共价化合物库 | EMA 上市药物库 | FDA 上市药物库 | 药物功能重定位化合物库 | 儿童药物库
Doxycycline hydrochloride hemiethanolate hemihydrate|||WC2031|||盐酸强力霉素|||Doxycycline hyclate

公司简介

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。 从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。 经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期 (12年)
注册资本 566.2651万人民币
员工人数 100-500人
年营业额 ￥ 1亿以上
经营模式 贸易,试剂,定制,服务
主营行业 化学试剂,生物活性小分子

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