重组人CD39蛋白-ACROBiosystems百普赛斯

Human CD39 / ENTPD1 Protein, His Tag (active enzyme, MALS verified)

分子别名（Synonym）

CD39,ENTPD1,NTPDase 1,Entpd1,Ecto-ATPDase 1,Ecto-ATPase 1

表达区间及表达系统（Source）

Human CD39, His Tag (CD9-H52H4) is expressed from human 293 cells (HEK293). It contains AA Thr 38 - Val 478 (Accession # P49961-1).

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 52.3 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in Tris and NaCl, pH8.0 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

CD39在神经系统中也被称为外核苷三磷酸二磷酸水解酶1、ENTPD1、NTPDase 1、外ATP酶1，可以水解ATP和其他核苷酸来调节嘌呤能神经传递。也可能通过将血小板活化ADP水解为AMP来预防血小板聚集。水解ATP和ADP同样好。NTPDase-1最初被描述为CD39，一种B淋巴细胞表面标志物，但它也存在于自然杀伤细胞、T细胞和一些内皮细胞的表面。调节性T细胞（Tregs）通过多种非冗余、细胞接触依赖和独立的机制介导免疫抑制，越来越多的证据表明CD39-CD73-腺苷途径起着重要作用。CD39外核苷酸酶是级联反应的限速酶，导致抑制性腺苷的产生，从而改变CD4和CD8 T细胞以及自然杀伤细胞的抗肿瘤活性。