重组人GAPDH酶-ACROBiosystems百普赛斯

Human GAPDH / G3PDH Protein, His Tag

分子别名（Synonym）

GAPDH,GAPD,CDABP0047,OK,SW-cl.12

表达区间及表达系统（Source）

Human GAPDH, His Tag (GAH-H5145) is expressed from E. coli cells. It contains AA Gly 2 - Glu 335 (Accession # AAH01601).

Predicted N-terminus: Met

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 36.9 kDa. The protein migrates as 37-38 kDa under reducing (R) condition (SDS-PAGE).

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

甘油醛-3-磷酸脱氢酶（GAPDH）也被称为肽基半胱氨酸S-亚硝酰酶GAPDH，它是一种催化糖酵解第六步的酶，因此用于分解葡萄糖以获得能量和碳分子。GAPDH具有甘油醛-3-磷酸脱氢酶和亚硝酰酶活性，因此分别在糖酵解和核功能中发挥作用。GAPDH可以参与核事件，包括转录、RNA转运、DNA复制和凋亡。此外，GAPDH可以调节细胞骨架的组织和组装。此外，GAPDH是GAIT（γ-干扰素激活的翻译抑制剂）复合物的一个组成部分，该复合物在炎症过程中介导干扰素-γ诱导的转录选择性翻译抑制。