重组人CTLA-4蛋白-ACROBiosystems百普赛斯

Human CTLA-4 / CD152 Protein, His Tag

分子别名（Synonym）

CTLA4,CD152

表达区间及表达系统（Source）

Human CTLA-4, His Tag (CT4-H5229) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Phe 162 (Accession # P16410-1).

Predicted N-terminus: Ala 37

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 15.4 kDa. The protein migrates as 20-25 kDa under reducing (R) condition (SDS-PAGE) due to different extent glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 6 months under sterile conditions after reconstitution.

背景（Background）

CTLA-4（细胞毒性T淋巴细胞抗原4）也称为CD152（分化簇152），是一种下调免疫系统的蛋白质受体。CTLA4是免疫球蛋白超家族的成员，在辅助T细胞表面表达，并向T细胞传递抑制信号。该蛋白质包含一个细胞外V结构域、一个跨膜结构域和一个细胞质尾部。编码不同异构体的替代剪接变体。CTLA4与T细胞共刺激蛋白CD28相似，两种分子都与抗原呈递细胞上的CD80和CD86（分别称为B7-1和B7-2）结合。CTLA4向T细胞传递抑制信号，而CD28则传递刺激信号。细胞内CTLA4也存在于调节性T细胞中，可能对其功能很重要。CTLA4和抗体的融合蛋白（CTLA4-Ig）已用于类风湿性关节炎的临床试验。