Human EGF Protein, His Tag, premium grade
分子别名（Synonym）
EGF,URG,HOMG4
表达区间及表达系统（Source）
Human EGF Protein, His Tag, premium grade (EGF-H52H3) is expressed from human 293 cells (HEK293). It contains AA Asn 971 - Arg 1023 (Accession # P01133-1).
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 8.3 kDa. The protein migrates as 10-11 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
无菌（Sterility）
Negative
支原体（Mycoplasma）
Negative.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景（Background）
人表皮生长因子（EGF）也称为HOMG4和URG，是一种生长因子，通过与其受体EGFR结合在调节细胞生长，增殖和分化中起重要作用。表皮生长因子可以在人血小板，巨噬细胞，尿液，唾液，牛奶和血浆中发现。EGF是EGF蛋白家族的创始成员。所有家族成员都包含一个或多个保守氨基酸序列的重复序列。唾液EGF的生物学效应包括口腔和胃食管溃疡的愈合，胃酸分泌的抑制，D合成的刺激以及粘膜保护免受腔内有害因素（如胃酸，胆汁酸，胃蛋白酶和胰蛋白酶）以及物理，化学和细菌剂。由于EGF会增加患癌症的风险，因此抑制它会降低患癌症的风险。
