MCP-1/CCL2 ELISA试剂盒-ACROBiosystems百普赛斯

ClinMax™ Human MCP-1 / CCL2 ELISA Kit

背景（Background）

ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human CCL2 that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.

A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.

ClinMax™ ELISA Kits are manufactured in a GMP-like facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.

原理（Assay Principles）

应用说明（Application）

The kit is developed for quantitative detection of human MCP-1/CCL2 in serum, plasma and cell culture supernates.

It is for research use only.

存储（Storage）

Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

背景（Background）：CCL2

Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. CCL2, a member of the CC subfamily, is characterized by two adjacent cysteine residues. CCL2 displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.