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网站主页 RECOMBINANT AKT1/AKT2/AKT3 ANTIBODY Recombinant AKT1/AKT2/AKT3 Antibody

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aladdin 阿拉丁 Ab087925 Recombinant AKT1/AKT2/AKT3 Antibody Recombinant (R04-8A4); Rabbit anti Human AKT1/AKT2/AKT3 Antibody; WB, IHC; Unconjugated

Recombinant AKT1/AKT2/AKT3 Antibody

￥499.90 10μl 起订

￥1299.90 50μl 起订

￥2099.90 100μl 起订

上海更新日期：2025-05-16

上海阿拉丁生化科技股份有限公司

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联系人：阿拉丁客服

电话：400-6206333拨打

手机：13167063860 拨打

邮箱：anhua.mao@aladdin-e.com

产品详情:

中文名称：: Recombinant AKT1/AKT2/AKT3 Antibody

英文名称：: Recombinant AKT1/AKT2/AKT3 Antibody

品牌：: 阿拉丁

产地：: 上海

保存条件：: -20°C储存,避免反复冻融

纯度规格：: 重组,ExactAb™,已验证,见COA

产品类别：: 抗体重组抗体

分子式：: C60H103N2O13S4

分子量：: 1286.75

运输条件：: 超低温运输

产品规格：: 100μl、50μl、10μl

货号：: Ab087925

是否进口：: 否

基本信息

产品名称：Recombinant AKT1/AKT2/AKT3 Antibody

规格或纯度：重组,ExactAb™,已验证,见COA

应用：WB, IF/ICC, IHC

特异性：AKT1/AKT2/AKT3

种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat), 仓鼠(Hamster)

免疫原：Recombinant protein of human AKT1/2/3 (AA 281-480).

阳性对照：WB: HeLa, Jurkat, NIH/3T3, A549 and MCF7 cell lysates. IHC: Human lung cancer tissue. IF/ICC: HeLa cells.

偶联：Unconjugated

产品属性

克隆类型：重组抗体

Format：Whole IgG

亚型：IgG

SDS-PAGE：150 kDa

纯化方法：Protein A purified

物理外观：Liquid

储存缓冲液：50mM Tris-Glycine, 150mM NaCl, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.4

防腐剂：0.01% Sodium azide

浓度：见COA

储存温度：-20°C储存,避免反复冻融

运输条件：超低温运输

稳定性与储存：短期储存2-8℃（1-2周）；长期储存-20℃（24个月）；收货后建议分装，避免反复冻融。

功能和特点

宿主种属：兔(Rabbit)

种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat), 仓鼠(Hamster)

偶联：Unconjugated

图片

Western Blot: Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925)

Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) - Western Blot
All lanes: Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 56 kDa
Observed band size: 57 kDa
Exposure time: 2.7 s

IHC: Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925)

Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925)

Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) - IF/ICC

IF analysis of Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).

IF/ICC: Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925)

Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) - IF/ICC

The HeLa cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubated with Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) (1/100) at room temperature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (shown in green). Nuclear DNA was labeled in blue with DAPI. Control: Secondary antibody only.

查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/Ab087925.html

AKT1/AKT2/AKT3抗体 ; 蛋白激酶AKT1/AKT2/AKT3抗体 ; 重组 AKT1/AKT2/AKT3 抗体 ; 蛋白激酶AKT1/2/3抗体

公司简介

上海阿拉丁生化科技股份有限公司是A股上市公司（（股票代码：688179），专注于科研试剂的研发、生产和销售，已陆续建立多个工厂和研发中心。作为领军企业，阿拉丁始终坚持质量第一，连续13年被评为“最受欢迎试剂品牌”。阿拉丁目前常备库存试剂产品品种超过7万种，SKU总数超过46万，产品线涵盖了化学试剂、生化试剂、药靶配体、蛋白质和抗体等多个领域，是国内少数化学试剂到生物试剂全面发展的国产试剂品牌，产品同步发布在我们国内外电商平台。

成立日期	(17年)
注册资本	14130.676万人民币
员工人数	500人以上
年营业额	￥ 1亿以上
经营模式	工厂,试剂
主营行业	生物化工,化学试剂

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