EphA2重组蛋白，ACROBiosystems百普赛斯

EphA2产品信息

英文名称：Ephrin type-A receptor 2

中文名称：A型肝配蛋白受体-2

靶点别称：EphA2

物种：Human

标签：His Tag & Avi Tag

属性：Protein

标记：Biotin-labeled

表达区间及表达系统（Source）

Biotinylated Human EphA2 Protein, His,Avitag (EP2-H82E4) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Asn 534 (Accession # P29317 ).

Predicted N-terminus: Ala 24

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

The protein has a calculated MW of 59.8 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

EphA2分子背景

受体酪氨酸激酶与位于邻近细胞上的膜结合的肝配蛋白-A家族配体混杂结合，导致接触依赖性双向信号传导进入邻近细胞。受体下游的信号通路称为正向信号通路，而肝配蛋白配体下游的信号途径称为反向信号通路。由配体ephrin-A1/EF1激活，调节细胞的迁移、整合素介导的粘附、增殖和分化。通过DSG1/桥粒糖蛋白-1和抑制ERK1/ERK2（分别为MAPK3/MAPK1）信号通路调节细胞粘附和分化。与配体ephrin-A5/EF5结合可以调节晶状体纤维细胞的形状和相互作用，对晶状体透明度的发展和维持很重要。ephrin-A2/EF2可能通过调节破骨细胞生成和成骨细胞生成在骨重塑中发挥作用。