NHS-dPEG <SUP>?</SUP> <SUB> 4 </SUB>-生物素,459426-22-3

一般描述

NHS-dPEG®4-biotin is a water-soluble cross-linking reagent used in biotinylation reactions. The N-hydroxysuccinimidyl (NHS) moiety of NHS-dPEG®4-biotin forms a stable amide bond with free primary amines, such as the amines on the side chain of lysine. The biotin moiety is used in a variety of applications, including labeling, supramolecular building, affinity chromatography, pull-down assays, and the production of biotinylated antibodies.

特点和优势

NHS-dPEG ® 4 -生物素被广泛用于标记含有游离胺的分子。单分子量 NHS-dPEG ® 4 -生物素在生物素部分和标记的分子之间有一段长度精确 (19.2 Å) 的长间隔区。该 dPEG ® 垫片使生物素标记物具有高度水溶性。对于生物素化的多肽和蛋白，dPEG ® 间隔物显著减少了修饰分子的聚集和沉淀，减少了分子的非特异性结合，这两者都是已经用广泛使用的 NHS-LC-biotin 生物素化的分子的常见问题。