血浆/血清RNA纯化中提试剂盒（提取试剂盒）

中文名称

血浆/血清RNA纯化中提试剂盒（提取试剂盒）

英文名字

Plasma/Serum RNA Purification Midi Kit

供应商

Norgen Biotek

产品货号

NGB-56100

产品报价

￥5166/20Preps

产品说明书

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购买方式

银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868。

产品新闻

背景资料

Plasma/Serum cell-free circulating RNA or exosomal RNA has the potential to provide biomarkers for certain cancers and disease states. Exosomes are 40 - 150 nm membrane vesicles, which are secreted by most cell types. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascitic fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. For this reason exosomal RNAs may serve as biomarkers for various diseases including cancer. As the RNA molecules encapsulated within exosomes are protected from degradation by RNAses they can be efficiently recovered from biological fluids, such as plasma or serum.

产品描述

This kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating and exosomal RNA using a two column method. This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 250 µL to 1.5 mL. Plasma samples prepared from blood collected on heparin should not be used, as heparin can significantly interfere with many downstream applications such as RT-PCR.The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL. All components for the purification and concentration are provided in one convenient and fast kit for the easy processing of large input volumes of bodily fluids. For a schematic workflow of the protocol click here.The purified plasma/serum RNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.

产品特点

Isolate all sizes of circulating and exosomal RNA, including microRNA; Versatile plasma/serum input ranging from 250 µL to 1.5 mL; No phenol extractions; No carrier RNA; Bind and elute all RNA irrespective of size or GC content, without bias; Concentrate circulating RNA and exosomal RNA into a flexible elution volume ranging from 50 µL to 100 µL; Purify high-quality RNA in 35-40 minutes.

保存建议

溶液密封室温(15-25°C)保存，未开封至少保存2年。具体保存情况收到产品后请按说明书存放。

其他

Norgen Biotek成立于1998年，公司总部位于加拿大，是一家著名全球化生物技术公司，基于专利的碳化硅膜纯化技术和无苯酚专利配方，生产高品质的核酸和蛋白质纯化及富集产品用于研究和诊断。Norgen Biotek 在2003年获得加拿大国家研究委员会颁发的科技创新奖，是一家致力于样品制备并获得ISO 9001（产品质量）、ISO 13485（产品可用于商业化的医疗设备，包括用于体外诊断领域）和ISO 15189（可用于临床检测机分子诊断领域的研发能力）认证的生物科技公司。 目前该公司的产品多达700余种，提供一流的样品制备试剂盒，应用于基因组和蛋白组等不同的领域。其产品主要覆盖包括各种标本类型的DNA，RNA和蛋白质的纯化富集，从活检样本（包括尿液，血浆，血清和血液）中纯化RNA和DNA，全系列的PCR试剂、缓冲液、分子量ladder和塑料过滤装置。