表皮转谷氨酰胺酶(TGM3)重组蛋白

表皮转谷氨酰胺酶(TGM3)重组蛋白

[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

Protein Protein Protein Protein Names: Transglutaminase 3, Epidermal

Synonyms: Synonyms: Synonyms: Synonyms: TGM3

Species: Species: Species: Species: Human

Size: 10µg

Source: Source: Source: Source: Escherichia coli-derived

[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]

Residues: Residues: Residues: Residues: Ala468~Glu693 (Accession # Q08188),

with N-terminal His-Tag. Grade & Purity: Purity: Purity: Purity: >95%, 28kDa as determined by

SDS-PAGE reducing conditions.

Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS, pH

7.4, containing 5% sucrose. Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by

the LAL method).

Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 26.3kDa

Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 5.5

Human TGM3

[ PREPARATION PREPARATION PREPARATION PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGS- ATS SMGLETEEQE PSIIGKLKVA GMLAVGKEVN LVLLLKNLSR

DTKTVTVNMT AWTIIYNGTL VHEVWKDSAT MSLDPEEEAE HPIKISYAQY EKYLKSDNMI

RITAVCKVPD ESEVVVERDI ILDNPTLTLE VLNEARVRKP VNVQMLFSNP LDEPVRDCVL

MVEGSGLLLG NLKIDVPTLG PKEGSRVRFD ILPSRSGTKQ LLADFSCNKF PAIKAMLSID

VAE

[ REFERENCES REFERENCES REFERENCES REFERENCES ]

1. Liu J., et al. (2012) Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi

26:101-103.

2. Zone J.J., et al. (2011) J. Immunol. 186:4474-4480.

3. Burkard T.R., et al. (2011) BMC Syst. Biol. 5:17-17. 4. Yamane A., et al. (2010) FEBS J. 277:3564-3574.

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