文章标题：EGCG Upregulates UCP3 Levels to Protect MIN6 Pancreatic Islet Cells from Interleukin-1β-Induced Apoptosis
影响因子：4.319
期刊：Drug Design Development and Therapy
作者列表：Xu Jia, Ziren Luo, Ying Gao, Hua Liu, Xinghai Liu, Wenli Mai, Hong Liu, Qian Zheng
发表时间：2022-10-31
DOI:10.2147/DDDT.S270345
主要研究成果：Abstract
Objective
The protective effects of epigallocatechin gallate (EGCG) on interleukin-1β (IL-1β)-induced apoptosis were investigated in murine MIN6 pancreatic β-cells. The role of uncoupling protein-3 (UCP3) signaling in this process was also explored.
Methods
After treatment with IL-1β and EGCG, cells were collected and analyzed. Cell viability was measured using the CCK8 assay and the function of β-cells was evaluated by analyzing insulin secretion. Detection of mitochondrial function in cells was performed by measuring mitochondrial membrane potential, the concentration of ATP and activity of ROS. Apoptosis was analyzed by Hochest33258 staining and flow cytometry. Expression levels of UCP3 were interrogated using immunohistochemistry, RT-PCR and Western blotting.
