文章标题：Rutaecarpine Inhibits Intimal Hyperplasia in A Balloon-Injured Rat Artery Model
作者列表：Xu Yang, Chen Xiu-ping, Zhang Feng, Hou Hua-hua, Zhang Jing-yi, Lin Shu-xian, Sun An-sheng
期刊：Chinese Journal of Integrative Medicine
发表时间：2017-9-1
影响因子：1.116
DOI:10.1007/s11655-017-2900-3
主要研究成果：Abstract
Objective
To investigate the effect and potential mechanisms of rutaecarpine (Rut) in a rat artery balloon-injury model.
Methods
The intimal hyperplasia model was established by rubbing the endothelia with a balloon catheter in the common carotid artery (CCA) of rats. Fifty rats were randomly divided into five groups, ie. sham, model, Rut (25, 50 and 75 mg/kg) with 10 rats of each group. The rats were treated with or without Rut (25, 50, 75 mg/kg) by intragastric administration for 14 consecutive days following injury. The morphological changes of the intima were evaluated by hematoxylin-eosin staining. The expressions of proliferating cell nuclear antigen (PCNA) and smooth muscle (SM) α-actin in the ateries were assayed by immunohistochemical staining. The mRNA expressions of c-myc, extracellular signal-regulated kinase 2 (ERK2), MAPK phosphatase-1 (MKP-1) and endothelial nitric oxide synthase (eNOS) were determined by real-time reverse transcription-polymerase chain reaction. The protein expressions of MKP-1 and phosphorylated ERK2 (p-ERK2) were examined by Western blotting. The plasma contents of nitric oxide (NO) and cyclic guanosine 3',5'-monophosphate (cGMP) were also determined.
