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How do I differentiate between apoptotic cells and necrotic cells when using Annexin V?

发布人:RD Systems

发布日期:2025/3/4 17:17:30

During early apoptosis phosphatidylserine is exposed on the cell surface. Annexin-V-FITC binds to phosphatidylserine and can be assayed by flow cytometry. Positive labeling is one to two logs higher than unlabeled cells. Propidium iodide can be added to the annexin-labeled cells to identify necrotic, late apoptotic, damaged, or dead cells exhibiting a compromised plasma membrane.

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