Sodium Deoxycholate (NaDOC) is a bile salt derivative with detergent-like properties, widely used in biochemical, pharmaceutical, and industrial applications due to its ability to solubilize lipids and disrupt cell membranes. Below are its key applications:
1. Biological & Biochemical Research
- Cell Lysis & Protein Extraction:
- Mild detergent for breaking cell membranes (e.g., in mitochondria/plasma membrane isolation).
- Solubilizes membrane proteins and lipoproteins.
- Nucleic Acid Purification:
- Removes lipids/proteins during DNA/RNA extraction (e.g., in plasmid prep kits).
- Viral Studies:
- Disrupts viral envelopes (e.g., influenza, hepatitis B) for structural analysis.
2. Pharmaceutical & Medical Uses
- Drug Delivery:
- Enhances absorption of hydrophobic drugs (e.g., anticancer agents).
- Antimicrobial Agent:
- Disrupts bacterial membranes (limited use in topical formulations).
- Cholesterol & Gallstone Research:
- Mimics bile composition to study cholesterol crystallization.
3. Diagnostic & Laboratory Reagents
- Immunoassays (ELISA/Western Blot):
- Reduces nonspecific binding in antibody-based assays.
- Protein Crystallization:
- Additive to optimize crystal formation.
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