| Name | RG108 |
| Description | RG108 (N-Phthalyl-L-tryptophan) is a DNA methyltransferase inhibitor with an IC50 of 115 nM. |
| Cell Research | For the determination of cellular growth and viability, cells are stained with trypan blue and counted using a standard counting grid.(Only for Reference) |
| Kinase Assay | In vitro methylation assay: The substrate DNA for the in vitro methylation assay is a 798 bp fragment (?423/+375 relative to the initiation codon) from the promoter region of the human p16Ink4a gene. The methylation reaction contains 350 to 400 ng substrate DNA and 4 units of M.SssI methylase (0.5 μM) in a final volume of 50 μL. Inhibitors are added to final concentrations of 10, 100, 200, and 500 μM, respectively. Reactions are done at 37 °C for 2 hours. After completion, the reaction is inactivated at 65 °C for 15 minutes and the DNA is purified using PCR Purification kit. Three hundred nanograms of purified DNA is digested for 3 hours at 60 °C with 30 units of BstUI and analyzed on 2% Tris-borate EDTA agarose gels. |
| In vivo | In iPS cells derived from SM, RG108 significantly reduces DNA activity. In tumor cells, RG108 inhibits gene demethylation and reactivation without affecting the methylation of telomeric gene sequences. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | Ethanol : 33.4 mg/mL (100 mM)
DMSO : 40 mg/mL (119.64 mM)
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| Keywords | inhibit | DNA Methyltransferase | Inhibitor | DNMTs | DNA MTases | RG 108 | RG108 | RG-108 |
| Inhibitors Related | GSK-3484862 | Levetiracetam | Dihydro-5-azacytidine FA | 5-Azacytidine | Procainamide hydrochloride | SW155246 | Zebularine | γ-Oryzanol | TFMB-(S)-2-HG | 6-Thioguanine | 5-Methyl-2'-deoxycytidine | Hinokitiol |
| Related Compound Libraries | Methylation Compound Library | Target-Focused Phenotypic Screening Library | Bioactive Compound Library | Epigenetics Compound Library | Chromatin Modification Compound Library | Inhibitor Library | NO PAINS Compound Library | Stem Cell Differentiation Compound Library | Anti-Aging Compound Library | Bioactive Compounds Library Max |
United States
