Name | Puromycin dihydrochloride |
Description | Puromycin dihydrochloride (CL13900 dihydrochloride) is a cinnamamide adenosine antibiotic and an inhibitor of protein synthesis. Puromycin dihydrochloride inhibits protein synthesis by binding to RNA and has antitumor and antitrypanosomal activity. |
In vitro | METHODS: Human hepatocellular carcinoma cells, HepG2, and primary rat hepatocytes, PRH, were treated with Puromycin dihydrochloride (0-300 µmol/L) for 72 h, and cell growth inhibition was detected by MTT.
RESULTS: Puromycin dihydrochloride dose-dependently inhibited the growth of HepG2 and PRH cells, with IC50s of 1600 μmol/L and 2000 nmol/L, respectively.[1]
METHODS: Human breast cancer cells MDA-MB-231 and MDA-MB-436 were transfected with shRNAs-Puro lentivirus, and after 24 h, the transfected cells were cultured in new medium containing Puromycin dihydrochloride (1 μg/ml) for about 12 days, and then the cells were screened for the success of transfection.
RESULTS: Puromycin dihydrochloride screened the lentivirally transfected cells successfully. [2]
METHODS: Cells transfected with lentivirus were cultured in medium supplemented with Puromycin dihydrochloride (2 μg/mL).
RESULTS: Puromycin dihydrochloride screened and maintained lentivirally successfully transfected cells. [3] |
In vivo | Puromycin is utilized to select recombinant cells from non-cultured cells. It serves as a tool for studying protein synthesis in many systems. |
Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
Solubility Information | H2O : 92 mg/mL (169 mM) Ethanol : < 1 mg/mL (insoluble or slightly soluble) DMSO : 60 mg/mL (110.2 mM)
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Keywords | Antibiotic | Puromycin | Bacterial | Inhibitor | Puromycin Dihydrochloride | inhibit | CL 13900 Dihydrochloride | CL-13900 | CL-13900 Dihydrochloride | CL 13900 | Puromycin dihydrochloride | CL13900 Dihydrochloride | CL13900 |
Inhibitors Related | Neomycin sulfate | Dehydroacetic acid sodium | 5-Fluorouracil | Ampicillin sodium | Methyl anthranilate | Kanamycin sulfate | Sulfamethoxazole sodium | Guanidine hydrochloride | Doxycycline | EDTA copper(II) disodium salt | Thymidine | Dimethyl sulfoxide |
Related Compound Libraries | Anti-Tumor Natural Product Library | Anti-Parasitic Compound Library | Anti-Viral Compound Library | Antiparasitic Natural Product Library | Microbial Natural Product Library | Anti-Aging Compound Library | Anti-infective Natural Product Library | Anti-Cancer Active Compound Library |