Protease K is mainly used to remove DNase and RNA enzyme during nucleic acid extraction, remove histones bound to genome, and inactivate alkaline phosphatase. At present, molecular biology technology has been applied in many detection standards, such as transgenic detection, animal attribute detection, pathogenic microorganism detection, etc. In molecular biology detection, the quality of nucleic acid extraction directly affects the amplification efficiency of subsequent PCR reaction. Many standards have set up the digestion step of adding proteinase K in the nucleic acid extraction step to extract more pure nucleic acid. In order to protect nucleic acids, DNA extraction experiments in molecular biology usually contain reagents such as EDTA. Because EDTA can be complexed with metal ions, it may affect the enzyme activity; And it is usually digested at pH 8.0 and 55 ℃. Different pH values and temperature conditions will affect the enzyme activity.
It inactivates nucleotidase proteins during isolation of DNA and RNA. Remove the restriction of endotoxin on cationic protein, such as lysozyme and ribonuclease. The research report shows that the enzymes on the cell membrane used for separating liver, yeast and mung bean mitochondria are used for antibody labeling, and the antigen binding sites are exposed in paraffin embedded tissue sections. Digest the protein in brain tissue samples for the study of prion transmissible spongiform encephalopathy (TSE).
Storage condition: 2-8 ° C.
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