HEPES (4-hydroxyethyl piperazine ethanesulfonic acid) is a non-ionic amphoteric buffer with high polarity. It is inert to a variety of chemical reagents and enzymes, does not participate in and interfere with the process of biochemical reactions, and has no inhibition on enzyme chemical reactions. Therefore, it can be specially used for the research of organelles, highly variable and pH sensitive proteins and enzymes. Used as biological buffer for biochemical reagent; The reaction buffer, pre hybridization buffer and hybridization buffer for separating and analyzing RNA nuclear components; For RNA and T4RNA. Molecular biology grade is used for RNA 3 '- terminal labeling with T4RNA ligase, separation and analysis of reaction buffer components of nuclear RNA, and pre hybridization buffer and mild hybridization buffer components. Cell cell adhesion, short-term cell aggregation and culture, buffer used for cleaning tissues and cells.