| Name | Perifosine |
| Description | Perifosine (KRX-0401) is an oral-active, alkyl-phosphocholine Akt inhibitor with potential antineoplastic activity. |
| Cell Research | Cells are incubated in the medium with 10% FCS for 48 hours with indicated concentration of Periosine. Cell viability is determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. (Cell Proliferation Kit I; Roche). The absorbance at 590 nm is recorded using the 96-well plate reader.(Only for Reference) |
| Kinase Assay | Akt kinase assay: MM.1S cells are cultured in the presence or absence of perifosine (5 μM, 6 hours) and then stimulated with IL-6 (20 ng/mL, 10 minutes). In vitro akt kinase assay is then carried out using the Akt Kinase Assay Kit. |
| In vitro | In MM.1S cells, Perifosine (10 μM) completely inhibited Akt phosphorylation. In immortalized keratinocytes and head and neck squamous carcinoma cells (IC50=0.6-8.9 μM), Perifosine inhibited cell proliferation. In mouse glial progenitor cells, Perifosine induced cell cycle arrest at G1 and G2 by reducing the phosphorylation level of Akt and extracellular signal-regulated kinases, causing cell growth inhibition. In human hepatocellular carcinoma cells, Perifosine induced cell cycle arrest and apoptosis by blocking Akt phosphorylation. |
| In vivo | In MM.1S cells, Perifosine (10 μM) completely inhibited Akt phosphorylation. In immortalized keratinocytes and head and neck squamous carcinoma cells (IC50=0.6-8.9 μM), Perifosine inhibited cell proliferation. In mouse glial progenitor cells, Perifosine induced cell cycle arrest at G1 and G2 by reducing the phosphorylation level of Akt and extracellular signal-regulated kinases, causing cell growth inhibition. In human hepatocellular carcinoma cells, Perifosine induced cell cycle arrest and apoptosis by blocking Akt phosphorylation. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : Insoluble
H2O : 10 mM
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| Keywords | Protein kinase B | Inhibitor | Perifosine | NSC639966 | KRX 0401 | KRX0401 | inhibit | PKB | Apoptosis | D 21266 | NSC-639966 | D-21266 | Akt | Autophagy |
| Inhibitors Related | Stavudine | Sodium 4-phenylbutyrate | L-Ascorbic acid | Hydroxychloroquine | Guanidine hydrochloride | Tributyrin | Paeonol | Naringin |
| Related Compound Libraries | Bioactive Compound Library | Kinase Inhibitor Library | Anti-Ovarian Cancer Compound Library | Anti-Cancer Clinical Compound Library | Drug Repurposing Compound Library | Inhibitor Library | Anti-Aging Compound Library | Bioactive Compounds Library Max | Anti-Cancer Active Compound Library | Anti-Cancer Drug Library |
United States
