| 名称 | Oligomycin A |
| 描述 | Oligomycin A (MCH 32) is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria. |
| 细胞实验 | ATP and Oligomycin Dose-Response Growth Measurement. Cellular ATP changes are measured by CellTiter-Glo reagent. To measure oligomycin dose-response curves, the cells are plated in 96-well plates at about 400–500 cells/well in 100 μl of culture, dosed the next day, and grown for 4 additional days followed by assaying with Cell-Titer-Glo reagent. The dose-response curves are plotted with nonlinear regression analysis of GraphPad Prism.(Only for Reference) |
| 体外活性 | METHODS: Human melanoma cells WM3734 were treated with Oligomycin A (0.01-1 µg/mL) for 24 h, and lactate production was measured in culture supernatants.
RESULTS: Oligomycin A caused an increase in lactate production in WM3734 cells. [1]
METHODS: Human lung cancer cells H1299 were treated with Oligomycin A (0.1-1000 ng/mL) for 100 min, and cellular respiration was measured using Oxygen Biosensor System plates.
RESULTS: At 100 and 1000 ng/mL, Oligomycin A completely inhibited cellular respiration within approximately one hour. [2] |
| 体内活性 | METHODS: To assay anti-inflammatory activity, Oligomycin A (0.25 mg/kg) was injected intraperitoneally into C57BL6 mice in a psoriasis model once daily for five days.
RESULTS: Oligomycin A reduced ear thickness, keratinocyte proliferation and immune cell infiltration. [3] |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | DMSO : 10 mg/mL (12.64 mM)
Ethanol : < 1 mg/mL (insoluble or slightly soluble)
H2O : < 1 mg/mL (insoluble or slightly soluble)
|
| 关键字 | ATP Synthase | Fungal | MCH32 | Antibiotic | Oligomycin A | Inhibitor | MCH-32 | inhibit |
| 相关产品 | Dehydroacetic acid sodium | Chlorobutanol hemihydrate | Potassium gluconate | Chitosan (MW 150000) | Ampicillin sodium | Doxycycline (hyclate) | Cefaclor monohydrate | Sulfamethoxazole sodium | Doxycycline | Lauryl betaine | EDTA copper(II) disodium salt | Oleic acid |
| 相关库 | 抗癌天然产物库 | 经典已知活性库 | 膜蛋白靶向化合物库 | 稀有天然产物库 | 抗真菌库 | 抑制剂库 | 微生物天然产物库 | 高通量筛选天然产物库 | 抗感染天然产物库 | 离子通道库 |
United States
