| Name | Oligomycin A |
| Description | Oligomycin A (MCH 32) is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria. |
| Cell Research | ATP and Oligomycin Dose-Response Growth Measurement. Cellular ATP changes are measured by CellTiter-Glo reagent. To measure oligomycin dose-response curves, the cells are plated in 96-well plates at about 400–500 cells/well in 100 μl of culture, dosed the next day, and grown for 4 additional days followed by assaying with Cell-Titer-Glo reagent. The dose-response curves are plotted with nonlinear regression analysis of GraphPad Prism.(Only for Reference) |
| In vitro | METHODS: Human melanoma cells WM3734 were treated with Oligomycin A (0.01-1 µg/mL) for 24 h, and lactate production was measured in culture supernatants.
RESULTS: Oligomycin A caused an increase in lactate production in WM3734 cells. [1]
METHODS: Human lung cancer cells H1299 were treated with Oligomycin A (0.1-1000 ng/mL) for 100 min, and cellular respiration was measured using Oxygen Biosensor System plates.
RESULTS: At 100 and 1000 ng/mL, Oligomycin A completely inhibited cellular respiration within approximately one hour. [2] |
| In vivo | METHODS: To assay anti-inflammatory activity, Oligomycin A (0.25 mg/kg) was injected intraperitoneally into C57BL6 mice in a psoriasis model once daily for five days.
RESULTS: Oligomycin A reduced ear thickness, keratinocyte proliferation and immune cell infiltration. [3] |
| Storage | store at low temperature,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 1 mg/mL (1.26 mM), Sonication is recommended.
DMSO : 10 mg/mL (12.64 mM), Sonication is recommended.
Ethanol : < 1 mg/mL (insoluble or slightly soluble)
H2O : < 1 mg/mL (insoluble or slightly soluble)
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| Keywords | Oligomycin A | MCH-32 | MCH32 | Inhibitor | inhibit | Fungal | ATP Synthase | ATP synthase | Antibiotic |
| Inhibitors Related | Neomycin sulfate | Dehydroacetic acid sodium | L-Lactic acid | Potassium gluconate | Ampicillin sodium | Doxycycline (hyclate) | Kanamycin sulfate | Sulfamethoxazole sodium | Metronidazole | Doxycycline | Geraniol | EDTA copper(II) disodium salt |
| Related Compound Libraries | Anti-Tumor Natural Product Library | Apoptosis Compound Library | Macrocyclic Compound Library | Bioactive Compound Library | Rare Natural Product Library | Membrane Protein-targeted Compound Library | Antibiotics Library | Inhibitor Library | Microbial Natural Product Library | Natural Product Library for HTS | Anti-infective Natural Product Library | Bioactive Compounds Library Max |
United States
