| Name | N-Ethylmaleimide |
| Description | N-Ethylmaleimide (NEM) is a reagent for alkylation of free sulfhydryl groups, a cysteine protease inhibitor used in experimental biochemistry. N-Ethylmaleimide is also a deubiquitinating enzyme inhibitor that specifically inhibits phosphate transport in mitochondria. |
| In vitro | METHODS: Growth-arrested VSMC cells were treated with N-Ethylmaleimide (20 µM) and PDGF-BB (20 ng/mL) for 5 min - 2 h, and the expression levels of target proteins were detected by Western Blot.
RESULTS: N-Ethylmaleimide inhibited PDGF BB-stimulated Akt phosphorylation. [1]
METHODS: Jurkat T cells were treated with N-Ethylmaleimide (3-100 µM) for 1-20 min, and target protein expression levels were detected by immunoblots.
RESULTS: Treatment of intact cells with 50-100 µM N-Ethylmaleimide for 5-10 min resulted in a significant increase in tyrosine residue phosphorylation. [2] |
| In vivo | METHODS: To examine the potential enhancement of sleep properties induced by alprazolam (Alp), N-Ethylmaleimide (1 mg/kg, 1% CMC-Na) and Alp (1.84 mg/kg, 1% CMC-Na) were administered by gavage to C57BL/6J mice.
RESULTS: Combined administration of Alp and NEM significantly reduced sleep latency and prolonged sleep duration compared with Alp alone. This effect was characterized by a significant increase in REM duration. [3] |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | 5% DMSO+95% Saline : 1.2 mg/mL (9.59 mM), Solution.
DMSO : 237.5 mg/mL (1898.03 mM), Sonication is recommended.
H2O : 50 mg/mL (399.58 mM), Sonication and heating are recommended.
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| Keywords | VSMC | sulfhydryl | phosphate transport | N-Ethylmaleimide | NEthylmaleimide | N Ethylmaleimide | modify | Inhibitor | inhibit | DUBs | Deubiquitinase | CysteineProtease | cysteine residue | Cysteine protease | Cysteine Protease | Cathepsin | Apoptosis |
| Inhibitors Related | Stavudine | Aceglutamide | Urea | Cysteamine hydrochloride | Sodium 4-phenylbutyrate | Metronidazole | Citric Acid Triammonium | L-Methionine | Sodium butanoate | Dimethyl phthalate | Alginic acid | Dextran sulfate sodium salt (MW 5000) |
| Related Compound Libraries | Highly Selective Inhibitor Library | FDA-Approved & Pharmacopeia Drug Library | Target-Focused Phenotypic Screening Library | Bioactive Compound Library | Membrane Protein-targeted Compound Library | Tobacco Monomer Library | Inhibitor Library | Mitochondria-Targeted Compound Library | Anti-Aging Compound Library | Bioactive Compounds Library Max | Covalent Inhibitor Library | Anti-Cancer Compound Library |
United States
