| Name | Myricetin |
| Description | Myricetin (Cannabiscetin) is a natural flavonoid and MEK1 inhibitor. Myricetin has hypoglycemic, antioxidant, hepatoprotective, anti-tumor and anti-inflammatory activities. |
| Cell Research | Pancreatic cancer cells (MIA PaCa-2, Panc-1 or S2-013) or normal pancreatic ductal cells (PDCs) are treated with myricetin (12.5–200 μM). Cell viability is determined using the Dojindo Cell Counting Kit-8. Cells are seeded onto a 96-well plate at 1×104 cells per well and allowed to adhere overnight. After treatment with myricetin at various concentrations for 24 hours, 10 μL of the tetrazolium substrate is added to each well of the plate. Plates are incubated at 37°C for 1 hour, after which the absorbance at 450 nm is measured[2]. |
| In vitro | METHODS: Human hepatocellular carcinoma cells HepG2 and Huh-7 were treated with Myricetin (100-200 µM) for 72 h. Cell viability was measured by CCK-8 assay.
RESULTS: Myricetin significantly inhibited cell growth in a dose-dependent manner. [1]
METHODS: Ovarian cancer cells A2780 and OVCAR3 were treated with Myricetin (25 µM) for 48 h. Apoptosis was detected by single stranded-DNA Apoptosis ELISA kit.
RESULTS: In A2780 cells treated with Myricetin, an approximately 2.5-fold increase in apoptotic signaling was observed compared to untreated cells, whereas under similar treatment conditions, apoptotic signaling in OVCAR3 cells increased approximately 4-fold compared to untreated cells. [2] |
| In vivo | METHODS: To detect anti-tumor activity in vivo, Myricetin (30 mg/kg once a day) and cisplatin (5 mg/kg every three days) were intraperitoneally injected into BALB/c nude mice bearing Huh-7 xenografts for two weeks.
RESULTS: Treatment with Myricetin or cisplatin alone moderately inhibited tumor growth, but the combination treatment inhibited tumor growth more significantly than Myricetin or cisplatin alone. [1] |
| Storage | keep away from direct sunlight,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | Ethanol : 20 mg/mL (62.84 mM), Heating is recommended.
DMSO : 55 mg/mL (172.83 mM)
H2O : < 1 mg/mL (insoluble or slightly soluble)
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 6.3 mg/mL (19.8 mM), Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately.
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| Keywords | Inhibitor | inhibit | Myricetin | Endogenous Metabolite | Apoptosis | Autophagy |
| Inhibitors Related | Sucrose | Hydroxychloroquine | Daidzein | Guanidine hydrochloride | Tributyrin | Ferulic Acid | Paeonol | Glycerol | Thymidine |
| Related Compound Libraries | 抗癌天然产物库 | 中药单体化合物库 | 经典已知活性库 | 激酶抑制剂库 | 酪氨酸激酶分子库 | 苗药化合物库 | 高通量筛选天然产物库 | 化妆品成分化合物库 | 古代经典名方目录分子库 | 抗癌活性化合物库 |
United States
